In this work, TiO2 nanowires synthesized from a wet-corrosion process were presented for peptide sequencing by photocatalytic reaction with UV radiation. For the photocatalytic decomposition of peptides, the peptide sample was dropped on a target plate containing synthesized TiO2 nanowire zones and UV-irradiated. Subsequently, the target plate was analyzed by laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry using the synthesized TiO2 nanowires as a solid matrix. The feasibility of peptide sequencing based on the photocatalytic reaction with the synthesized TiO2 nanowires was demonstrated using six types of peptides GHP9 (G1-H-P-Q-G2-K1-K2-K3-K4, 1006.59 Da), BPA-1(K1-S1-L-E-N-S2-Y-G1-G2-G3-K2-K3-K4, 1394.74 Da), PreS1(F1-G-A-N1-S-N2-N3-P1-D1-W-D2-F2-N4-P2-N5, 1707.68 Da), HPQ peptide-1 (G-Y-H-P-Q-R-K, 884.45 Da), HPQ peptide-2 (K-R-H-P-Q-Y-G, 884.45 Da), and HPQ peptide-3 (R-Y-H-P-Q-G-K, 884.45 Da). The identification of three different peptides with the same molecular weight was also demonstrated by using the synthesized TiO2 nanowires for their photocatalytic decomposition as well as for LDI-TOF mass spectrometry as a solid-matrix.
All Science Journal Classification (ASJC) codes
- Materials Science(all)