TIP120A associates with cullins and modulates ubiquitin ligase activity

Kyoeng Woo Min, Ji Won Hwang, Jong Sik Lee, Yoon Park, Taka aki Tamura, Jong-Bok Yoon

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The cullin-containing ubiquitin-protein isopeptide ligases (E3s) play an important role in regulating the abundance of key proteins involved in cellular processes such as cell cycle and cytokine signaling. They have multisubunit modular structures in which substrate recognition and the catalysis of ubiquitination are carried out by distinct polypeptides. In a search for proteins involved in regulation of cullin-containing E3 ubiquitin ligases we immunopurified CUL4B-containing complex from HeLa cells and identified TIP120A as an associated protein by mass spectrometry. Immunoprecipitation of cullins revealed that all cullins tested specifically interacted with TIP120A. Reciprocal immunoaffinity purification of TIP120A confirmed the stable interaction of TIP120A with cullin family proteins. TIP120A formed a complex with CUL1 and Rbx1, but interfered with the binding of Skp1 and F-box proteins to CUL1. TIP120A greatly reduced the ubiquitination of phosphorylated IκBα by SCFβ-TrCP ubiquitin ligase. These results suggest that TIP120A functions as a negative regulator of SCF E3 ubiquitin ligases and may modulate other cullin ligases in a similar fashion.

Original languageEnglish
Pages (from-to)15905-15910
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number18
DOIs
Publication statusPublished - 2003 May 2

Fingerprint

Cullin Proteins
Ligases
Ubiquitin
Ubiquitin-Protein Ligases
SKP Cullin F-Box Protein Ligases
Ubiquitination
Proteins
F-Box Proteins
Catalysis
HeLa Cells
Immunoprecipitation
Purification
Mass spectrometry
Mass Spectrometry
Cell Cycle
Cells
Cytokines
Peptides
Substrates

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Min, Kyoeng Woo ; Hwang, Ji Won ; Lee, Jong Sik ; Park, Yoon ; Tamura, Taka aki ; Yoon, Jong-Bok. / TIP120A associates with cullins and modulates ubiquitin ligase activity. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 18. pp. 15905-15910.
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TIP120A associates with cullins and modulates ubiquitin ligase activity. / Min, Kyoeng Woo; Hwang, Ji Won; Lee, Jong Sik; Park, Yoon; Tamura, Taka aki; Yoon, Jong-Bok.

In: Journal of Biological Chemistry, Vol. 278, No. 18, 02.05.2003, p. 15905-15910.

Research output: Contribution to journalArticle

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