TIP120A associates with unneddylated cullin 1 and regulates its neddylation

Ji Won Hwang, Kyoeng Woo Min, Taka Aki Tamura, Jong-Bok Yoon

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The cullin-containing E3 ubiquitin ligases play an important role in regulating the abundance of key proteins involved in cellular processes such as cell cycle and cytokine signaling. We recently identified TIP120A as a cullin-interacting protein and found that TIP120A functions as a negative regulator of a ubiquitin ligase by interfering with the binding of Skp1 and an F box protein to CUL1. Here we show that TIP120A binds to the unneddylated CUL1 but not the neddylated one. The association of TIP120A with CUL1 requires both the N-terminal stalk and the C-terminal globular domain of CUL1. TIP120A efficiently inhibits neddylation of CUL1 but does not affect substrate-independent ubiquitination by CUL1/Rbx1, implying that it blocks the access of Nedd8 to the conjugation site but does not interfere with the interaction of the ubiquitin-conjugating enzyme with Rbx1. Our data suggest that the association/dissociation of TIP120A coupled to neddylation/deneddylation of CUL1 may play an important role in assembly and disassembly of Skp1-Cdc53/cullin-F box ubiquitin ligases.

Original languageEnglish
Pages (from-to)102-108
Number of pages7
JournalFEBS Letters
Volume541
Issue number1-3
DOIs
Publication statusPublished - 2003 Apr 24

Fingerprint

Cullin Proteins
Ligases
Ubiquitin
F-Box Proteins
Association reactions
Ubiquitin-Conjugating Enzymes
Ubiquitin-Protein Ligases
Ubiquitination
Cell Cycle
Proteins
Cells
Cytokines
Substrates
Cullin 1

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Hwang, Ji Won ; Min, Kyoeng Woo ; Tamura, Taka Aki ; Yoon, Jong-Bok. / TIP120A associates with unneddylated cullin 1 and regulates its neddylation. In: FEBS Letters. 2003 ; Vol. 541, No. 1-3. pp. 102-108.
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TIP120A associates with unneddylated cullin 1 and regulates its neddylation. / Hwang, Ji Won; Min, Kyoeng Woo; Tamura, Taka Aki; Yoon, Jong-Bok.

In: FEBS Letters, Vol. 541, No. 1-3, 24.04.2003, p. 102-108.

Research output: Contribution to journalArticle

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