Tissue Adhesive Catechol-Modified Hyaluronic Acid Hydrogel for Effective, Minimally Invasive Cell Therapy

Jisoo Shin, Jung Seung Lee, Changhyun Lee, Hyun Ji Park, Kisuk Yang, Yoonhee Jin, Ji Hyun Ryu, Ki Sung Hong, Sung Hwan Moon, Hyung Min Chung, Hee Seok Yang, Soong Ho Um, Jong-Won Oh, Dong Ik Kim, Haeshin Lee, Seung-Woo Cho

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Current hyaluronic acid (HA) hydrogel systems often cause cytotoxicity to encapsulated cells and lack the adhesive property required for effective localization of transplanted cells in vivo. In addition, the injection of hydrogel into certain organs (e.g., liver, heart) induces tissue damage and hemorrhage. In this study, we describe a bioinspired, tissue-adhesive hydrogel that overcomes the limitations of current HA hydrogels through its improved biocompatibility and potential for minimally invasive cell transplantation. HA functionalized with an adhesive catecholamine motif of mussel foot protein forms HA-catechol (HA-CA) hydrogel via oxidative crosslinking. HA-CA hydrogel increases viability, reduces apoptosis, and enhances the function of two types of cells (human adipose-derived stem cells and hepatocytes) compared with a typical HA hydrogel crosslinked by photopolymerization. Due to the strong tissue adhesiveness of the HA-CA hydrogel, cells are easily and efficiently transplanted onto various tissues (e.g., liver and heart) without the need for injection. Stem cell therapy using the HA-CA hydrogel increases angiogenesis in vivo, leading to improved treatment of ischemic diseases. HA-CA hydrogel also improved hepatic functions of transplanted hepatocytes in vivo. Thus, this bioinspired, tissue-adhesive HA hydrogel can enhance the efficacy of minimally invasive cell therapy.

Original languageEnglish
Pages (from-to)3814-3824
Number of pages11
JournalAdvanced Functional Materials
Volume25
Issue number25
DOIs
Publication statusPublished - 2015 Jul 1

Fingerprint

Tissue Adhesives
Hyaluronic acid
Hydrogel
Hyaluronic Acid
Hydrogels
adhesives
therapy
Tissue
acids
cells
Adhesives
stem cells
Stem cells
liver
Liver
catecholamine
catechol
injection
hemorrhages
angiogenesis

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Condensed Matter Physics
  • Electrochemistry

Cite this

Shin, Jisoo ; Lee, Jung Seung ; Lee, Changhyun ; Park, Hyun Ji ; Yang, Kisuk ; Jin, Yoonhee ; Ryu, Ji Hyun ; Hong, Ki Sung ; Moon, Sung Hwan ; Chung, Hyung Min ; Yang, Hee Seok ; Um, Soong Ho ; Oh, Jong-Won ; Kim, Dong Ik ; Lee, Haeshin ; Cho, Seung-Woo. / Tissue Adhesive Catechol-Modified Hyaluronic Acid Hydrogel for Effective, Minimally Invasive Cell Therapy. In: Advanced Functional Materials. 2015 ; Vol. 25, No. 25. pp. 3814-3824.
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abstract = "Current hyaluronic acid (HA) hydrogel systems often cause cytotoxicity to encapsulated cells and lack the adhesive property required for effective localization of transplanted cells in vivo. In addition, the injection of hydrogel into certain organs (e.g., liver, heart) induces tissue damage and hemorrhage. In this study, we describe a bioinspired, tissue-adhesive hydrogel that overcomes the limitations of current HA hydrogels through its improved biocompatibility and potential for minimally invasive cell transplantation. HA functionalized with an adhesive catecholamine motif of mussel foot protein forms HA-catechol (HA-CA) hydrogel via oxidative crosslinking. HA-CA hydrogel increases viability, reduces apoptosis, and enhances the function of two types of cells (human adipose-derived stem cells and hepatocytes) compared with a typical HA hydrogel crosslinked by photopolymerization. Due to the strong tissue adhesiveness of the HA-CA hydrogel, cells are easily and efficiently transplanted onto various tissues (e.g., liver and heart) without the need for injection. Stem cell therapy using the HA-CA hydrogel increases angiogenesis in vivo, leading to improved treatment of ischemic diseases. HA-CA hydrogel also improved hepatic functions of transplanted hepatocytes in vivo. Thus, this bioinspired, tissue-adhesive HA hydrogel can enhance the efficacy of minimally invasive cell therapy.",
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Shin, J, Lee, JS, Lee, C, Park, HJ, Yang, K, Jin, Y, Ryu, JH, Hong, KS, Moon, SH, Chung, HM, Yang, HS, Um, SH, Oh, J-W, Kim, DI, Lee, H & Cho, S-W 2015, 'Tissue Adhesive Catechol-Modified Hyaluronic Acid Hydrogel for Effective, Minimally Invasive Cell Therapy', Advanced Functional Materials, vol. 25, no. 25, pp. 3814-3824. https://doi.org/10.1002/adfm.201500006

Tissue Adhesive Catechol-Modified Hyaluronic Acid Hydrogel for Effective, Minimally Invasive Cell Therapy. / Shin, Jisoo; Lee, Jung Seung; Lee, Changhyun; Park, Hyun Ji; Yang, Kisuk; Jin, Yoonhee; Ryu, Ji Hyun; Hong, Ki Sung; Moon, Sung Hwan; Chung, Hyung Min; Yang, Hee Seok; Um, Soong Ho; Oh, Jong-Won; Kim, Dong Ik; Lee, Haeshin; Cho, Seung-Woo.

In: Advanced Functional Materials, Vol. 25, No. 25, 01.07.2015, p. 3814-3824.

Research output: Contribution to journalArticle

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AU - Shin, Jisoo

AU - Lee, Jung Seung

AU - Lee, Changhyun

AU - Park, Hyun Ji

AU - Yang, Kisuk

AU - Jin, Yoonhee

AU - Ryu, Ji Hyun

AU - Hong, Ki Sung

AU - Moon, Sung Hwan

AU - Chung, Hyung Min

AU - Yang, Hee Seok

AU - Um, Soong Ho

AU - Oh, Jong-Won

AU - Kim, Dong Ik

AU - Lee, Haeshin

AU - Cho, Seung-Woo

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Current hyaluronic acid (HA) hydrogel systems often cause cytotoxicity to encapsulated cells and lack the adhesive property required for effective localization of transplanted cells in vivo. In addition, the injection of hydrogel into certain organs (e.g., liver, heart) induces tissue damage and hemorrhage. In this study, we describe a bioinspired, tissue-adhesive hydrogel that overcomes the limitations of current HA hydrogels through its improved biocompatibility and potential for minimally invasive cell transplantation. HA functionalized with an adhesive catecholamine motif of mussel foot protein forms HA-catechol (HA-CA) hydrogel via oxidative crosslinking. HA-CA hydrogel increases viability, reduces apoptosis, and enhances the function of two types of cells (human adipose-derived stem cells and hepatocytes) compared with a typical HA hydrogel crosslinked by photopolymerization. Due to the strong tissue adhesiveness of the HA-CA hydrogel, cells are easily and efficiently transplanted onto various tissues (e.g., liver and heart) without the need for injection. Stem cell therapy using the HA-CA hydrogel increases angiogenesis in vivo, leading to improved treatment of ischemic diseases. HA-CA hydrogel also improved hepatic functions of transplanted hepatocytes in vivo. Thus, this bioinspired, tissue-adhesive HA hydrogel can enhance the efficacy of minimally invasive cell therapy.

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