Tissue engineering of heart valves in vivo using bone marrow-derived cells

Sang Soo Kim; Sang Hyun Lim; Yoo Sun Hong; Seung Woo Cho; Ju Hee Ryu; Byung Chul Chang; Cha Yong Choi; Byung Soo Kim

doi:10.1111/j.1525-1594.2006.00258.x

Tissue engineering of heart valves in vivo using bone marrow-derived cells

Sang Soo Kim, Sang Hyun Lim, Yoo Sun Hong, Seung Woo Cho, Ju Hee Ryu, Byung Chul Chang, Cha Yong Choi, Byung Soo Kim

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

In this study, we tissue-engineered heart valves in vivo using autologous bone marrow-derived cells (BMCs). Canine BMCs were differentiated into endothelial cell (EC)-like cells and myofibroblast (MF)-like cells. Decellularized porcine pulmonary valves were seeded with BMCs and implanted to abdominal aorta and pulmonary valve of bone marrow donor dogs. Histological examination of the explants identified the regeneration of valvular structures expressing CD31 and smooth muscle α-actin, indicating the presence of EC-like and MF-like cells in the grafts at 3 and 1 week, respectively, after implantation. Fluorescent microscopic examinations identified the presence of fluorescently labeled cells in the explants, indicating that the implanted BMCs survived and participated in the heart valve reconstitution. This study reports, for the first time, on tissue engineering of heart valve in vivo using BMCs.

Original language	English
Pages (from-to)	554-557
Number of pages	4
Journal	Artificial Organs
Volume	30
Issue number	7
DOIs	https://doi.org/10.1111/j.1525-1594.2006.00258.x
Publication status	Published - 2006 Jul

All Science Journal Classification (ASJC) codes

Bioengineering
Medicine (miscellaneous)
Biomaterials
Biomedical Engineering

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10.1111/j.1525-1594.2006.00258.x

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title = "Tissue engineering of heart valves in vivo using bone marrow-derived cells",

abstract = "In this study, we tissue-engineered heart valves in vivo using autologous bone marrow-derived cells (BMCs). Canine BMCs were differentiated into endothelial cell (EC)-like cells and myofibroblast (MF)-like cells. Decellularized porcine pulmonary valves were seeded with BMCs and implanted to abdominal aorta and pulmonary valve of bone marrow donor dogs. Histological examination of the explants identified the regeneration of valvular structures expressing CD31 and smooth muscle α-actin, indicating the presence of EC-like and MF-like cells in the grafts at 3 and 1 week, respectively, after implantation. Fluorescent microscopic examinations identified the presence of fluorescently labeled cells in the explants, indicating that the implanted BMCs survived and participated in the heart valve reconstitution. This study reports, for the first time, on tissue engineering of heart valve in vivo using BMCs.",

author = "Kim, {Sang Soo} and Lim, {Sang Hyun} and Hong, {Yoo Sun} and Cho, {Seung Woo} and Ryu, {Ju Hee} and Chang, {Byung Chul} and Choi, {Cha Yong} and Kim, {Byung Soo}",

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AU - Kim, Sang Soo

AU - Lim, Sang Hyun

AU - Hong, Yoo Sun

AU - Cho, Seung Woo

AU - Ryu, Ju Hee

AU - Chang, Byung Chul

AU - Choi, Cha Yong

AU - Kim, Byung Soo

PY - 2006/7

Y1 - 2006/7

N2 - In this study, we tissue-engineered heart valves in vivo using autologous bone marrow-derived cells (BMCs). Canine BMCs were differentiated into endothelial cell (EC)-like cells and myofibroblast (MF)-like cells. Decellularized porcine pulmonary valves were seeded with BMCs and implanted to abdominal aorta and pulmonary valve of bone marrow donor dogs. Histological examination of the explants identified the regeneration of valvular structures expressing CD31 and smooth muscle α-actin, indicating the presence of EC-like and MF-like cells in the grafts at 3 and 1 week, respectively, after implantation. Fluorescent microscopic examinations identified the presence of fluorescently labeled cells in the explants, indicating that the implanted BMCs survived and participated in the heart valve reconstitution. This study reports, for the first time, on tissue engineering of heart valve in vivo using BMCs.

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Tissue engineering of heart valves in vivo using bone marrow-derived cells

Abstract

All Science Journal Classification (ASJC) codes

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