Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids

Suran Kim, Sungjin Min, Yi Sun Choi, Sung Hyun Jo, Jae Hun Jung, Kyusun Han, Jin Kim, Soohwan An, Yong Woo Ji, Yun Gon Kim, Seung Woo Cho

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Matrigel, a mouse tumor extracellular matrix protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to-batch variation, high cost, and safety issues. Here, we demonstrate that gastrointestinal tissue-derived extracellular matrix hydrogels are suitable substitutes for Matrigel in gastrointestinal organoid culture. We found that the development and function of gastric or intestinal organoids grown in tissue extracellular matrix hydrogels are comparable or often superior to those in Matrigel. In addition, gastrointestinal extracellular matrix hydrogels enabled long-term subculture and transplantation of organoids by providing gastrointestinal tissue-mimetic microenvironments. Tissue-specific and age-related extracellular matrix profiles that affect organoid development were also elucidated through proteomic analysis. Together, our results suggest that extracellular matrix hydrogels derived from decellularized gastrointestinal tissues are effective alternatives to the current gold standard, Matrigel, and produce organoids suitable for gastrointestinal disease modeling, drug development, and tissue regeneration.

Original languageEnglish
Article number1692
JournalNature communications
Issue number1
Publication statusPublished - 2022 Dec

Bibliographical note

Funding Information:
This work was supported by grants (2018M3A9H1021382 and 2021R1A2C3004262 to S.W.C.) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT), Republic of Korea. This work was also supported by a grant (20009125 to S.W.C.) from the Korea Evaluation Institute of Industrial Technology (KEIT) funded by the MSIT. This work was further supported by the Institute for Basic Science (IBS-R026-D1 to S.W.C.) and the Yonsei Signature Research Cluster Program (2021-22-0014 to S.W.C.). The authors sincerely thank Dr. Bon-Kyoung Koo (Institute of Molecular Biotechnology, Vienna, Austria) for helpful comments and discussion.

Publisher Copyright:
© 2022, The Author(s).

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • General
  • Physics and Astronomy(all)


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