Tissue transglutaminase 2 promotes apoptosis of rat neonatal cardiomyocytes under oxidative stress

Heesang Song, Byoung Keuk Kim, Woochul Chang, Soyeon Lim, Byeong Wook Song, Min Ji Cha, Yangsoo Jang, Ki Chul Hwang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The role of tissue transglutaminase 2 (TG2) in cardiac myocyte apoptosis under oxidative stress induced by ischemic injury remains unclear. Here, we investigated the effects of TG2 on apoptosis of cardiomyocytes under oxidative stress. Ectopic expression of TG2 increased caspase-3 activity and calcium overload in cardiomyocytes. Expression levels of TG2 were significantly increased in H2O2-treated cardiomyocytes. Caspase-3 activity assay demonstrated its considerable correlation with TG2 expression, which supported that caspase-3 inhibitor inhibited the apoptosis induced by the ectopic overexpression of TG2. In addition, the other apoptotic signals, such as caspase-8, cytochrome c, and Bax, were increased dependent with TG2 expression in H2O2-treated cardiomyocytes. These results indicated that apoptotic signals had a positive correlation with TG2 expression. The decreased expression of phospholipase C (PLC)-δ1 and phospho-PKC in H 2O2-treated cardiomyocytes were rescued by TG2 silencing. Together, our data strongly suggest that oxidative stress up-regulates TG2 expression in cardiomyocytes, leading to apoptosis.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalJournal of Receptors and Signal Transduction
Volume31
Issue number1
DOIs
Publication statusPublished - 2011 Feb 1

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Oxidative stress
Cardiac Myocytes
Rats
Oxidative Stress
Apoptosis
Caspase 3
transglutaminase 2
Caspase Inhibitors
Caspase 8
Type C Phospholipases
Cytochromes c
Assays
Up-Regulation
Calcium

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Song, Heesang ; Kim, Byoung Keuk ; Chang, Woochul ; Lim, Soyeon ; Song, Byeong Wook ; Cha, Min Ji ; Jang, Yangsoo ; Hwang, Ki Chul. / Tissue transglutaminase 2 promotes apoptosis of rat neonatal cardiomyocytes under oxidative stress. In: Journal of Receptors and Signal Transduction. 2011 ; Vol. 31, No. 1. pp. 66-74.
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Tissue transglutaminase 2 promotes apoptosis of rat neonatal cardiomyocytes under oxidative stress. / Song, Heesang; Kim, Byoung Keuk; Chang, Woochul; Lim, Soyeon; Song, Byeong Wook; Cha, Min Ji; Jang, Yangsoo; Hwang, Ki Chul.

In: Journal of Receptors and Signal Transduction, Vol. 31, No. 1, 01.02.2011, p. 66-74.

Research output: Contribution to journalArticle

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