Titanium-Silver Alloy Miniplates for Mandibular Fixation: In Vitro and In Vivo Study

Jung Hwan Lee, Jae Sung Kwon, Seung Kyun Moon, Soo Hyuk Uhm, Byungho Choi, Uk Hyon Joo, Kwangmahn Kim, Kyoung Nam Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose Titanium (Ti) alloys have received considerable attention as materials for oral and maxillofacial surgery, which require high mechanical strength, osteosynthesis, and biocompatibility. The objective was to implant miniplates fabricated from commercially pure Ti (CP Ti) and newly developed Ti–silver (Ag) alloy in fractured mandibles of adult dogs after preliminary mechanical and biological characterization. Materials and Methods The surface characteristics, biocompatibility, and pre-osteoblast adhesion and proliferation of CP Ti (grade 3) and Ti-Ag (2 at% Ag) alloys were evaluated. Next, the bending strength of 6- and 8-hole miniplates fabricated from CP Ti and Ti-Ag was compared according to ISO (International Organization for Standardization) 9585. Six-hole miniplates were implanted for 12 weeks in fractured mandibles of adult dogs. The Ag ion concentration in each alloy and implanted bone block with soft tissue was measured by inductively coupled plasma mass spectroscopy after euthanasia according to ISO 10993-12. Results Precipitated Ag was detected in Ti-Ag by alpha- and beta-phase Ti in x-ray powder diffraction. The biocompatibility with pre-osteoblasts of Ti-Ag and CP Ti was comparable in terms of cytotoxicity, cell adhesion, and proliferation (P > .05). Ti-Ag miniplates had up to 3-fold greater bending strength than CP Ti miniplates (P < .05). An in vivo study showed that CP Ti and Ti-Ag miniplates had comparable soft and hard tissue regeneration ability (P > .05). Ag ions were detected in Ti-Ag alloys and applied mandible blocks. Conclusions The results of this study suggest that Ti-Ag alloys can be used to produce miniplates with high mechanical properties, as well as considerable biocompatibility, osteosynthesis ability, and Ag ion–release properties. Further studies, including preclinical investigations, are required to enable clinical use of Ti-Ag bone plates.

Original languageEnglish
Pages (from-to)1622.e1-1622.e12
JournalJournal of Oral and Maxillofacial Surgery
Volume74
Issue number8
DOIs
Publication statusPublished - 2016 Aug 1

Fingerprint

Titanium
Silver
Mandible
Oral Surgery
Osteoblasts
In Vitro Techniques
Dogs
Ions
Bone Plates
Powder Diffraction
Euthanasia
Cell Adhesion
Mass Spectrometry
Cell Proliferation
X-Rays
Organizations
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

Cite this

Lee, Jung Hwan ; Kwon, Jae Sung ; Moon, Seung Kyun ; Uhm, Soo Hyuk ; Choi, Byungho ; Joo, Uk Hyon ; Kim, Kwangmahn ; Kim, Kyoung Nam. / Titanium-Silver Alloy Miniplates for Mandibular Fixation : In Vitro and In Vivo Study. In: Journal of Oral and Maxillofacial Surgery. 2016 ; Vol. 74, No. 8. pp. 1622.e1-1622.e12.
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abstract = "Purpose Titanium (Ti) alloys have received considerable attention as materials for oral and maxillofacial surgery, which require high mechanical strength, osteosynthesis, and biocompatibility. The objective was to implant miniplates fabricated from commercially pure Ti (CP Ti) and newly developed Ti–silver (Ag) alloy in fractured mandibles of adult dogs after preliminary mechanical and biological characterization. Materials and Methods The surface characteristics, biocompatibility, and pre-osteoblast adhesion and proliferation of CP Ti (grade 3) and Ti-Ag (2 at{\%} Ag) alloys were evaluated. Next, the bending strength of 6- and 8-hole miniplates fabricated from CP Ti and Ti-Ag was compared according to ISO (International Organization for Standardization) 9585. Six-hole miniplates were implanted for 12 weeks in fractured mandibles of adult dogs. The Ag ion concentration in each alloy and implanted bone block with soft tissue was measured by inductively coupled plasma mass spectroscopy after euthanasia according to ISO 10993-12. Results Precipitated Ag was detected in Ti-Ag by alpha- and beta-phase Ti in x-ray powder diffraction. The biocompatibility with pre-osteoblasts of Ti-Ag and CP Ti was comparable in terms of cytotoxicity, cell adhesion, and proliferation (P > .05). Ti-Ag miniplates had up to 3-fold greater bending strength than CP Ti miniplates (P < .05). An in vivo study showed that CP Ti and Ti-Ag miniplates had comparable soft and hard tissue regeneration ability (P > .05). Ag ions were detected in Ti-Ag alloys and applied mandible blocks. Conclusions The results of this study suggest that Ti-Ag alloys can be used to produce miniplates with high mechanical properties, as well as considerable biocompatibility, osteosynthesis ability, and Ag ion–release properties. Further studies, including preclinical investigations, are required to enable clinical use of Ti-Ag bone plates.",
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Titanium-Silver Alloy Miniplates for Mandibular Fixation : In Vitro and In Vivo Study. / Lee, Jung Hwan; Kwon, Jae Sung; Moon, Seung Kyun; Uhm, Soo Hyuk; Choi, Byungho; Joo, Uk Hyon; Kim, Kwangmahn; Kim, Kyoung Nam.

In: Journal of Oral and Maxillofacial Surgery, Vol. 74, No. 8, 01.08.2016, p. 1622.e1-1622.e12.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Titanium-Silver Alloy Miniplates for Mandibular Fixation

T2 - In Vitro and In Vivo Study

AU - Lee, Jung Hwan

AU - Kwon, Jae Sung

AU - Moon, Seung Kyun

AU - Uhm, Soo Hyuk

AU - Choi, Byungho

AU - Joo, Uk Hyon

AU - Kim, Kwangmahn

AU - Kim, Kyoung Nam

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Purpose Titanium (Ti) alloys have received considerable attention as materials for oral and maxillofacial surgery, which require high mechanical strength, osteosynthesis, and biocompatibility. The objective was to implant miniplates fabricated from commercially pure Ti (CP Ti) and newly developed Ti–silver (Ag) alloy in fractured mandibles of adult dogs after preliminary mechanical and biological characterization. Materials and Methods The surface characteristics, biocompatibility, and pre-osteoblast adhesion and proliferation of CP Ti (grade 3) and Ti-Ag (2 at% Ag) alloys were evaluated. Next, the bending strength of 6- and 8-hole miniplates fabricated from CP Ti and Ti-Ag was compared according to ISO (International Organization for Standardization) 9585. Six-hole miniplates were implanted for 12 weeks in fractured mandibles of adult dogs. The Ag ion concentration in each alloy and implanted bone block with soft tissue was measured by inductively coupled plasma mass spectroscopy after euthanasia according to ISO 10993-12. Results Precipitated Ag was detected in Ti-Ag by alpha- and beta-phase Ti in x-ray powder diffraction. The biocompatibility with pre-osteoblasts of Ti-Ag and CP Ti was comparable in terms of cytotoxicity, cell adhesion, and proliferation (P > .05). Ti-Ag miniplates had up to 3-fold greater bending strength than CP Ti miniplates (P < .05). An in vivo study showed that CP Ti and Ti-Ag miniplates had comparable soft and hard tissue regeneration ability (P > .05). Ag ions were detected in Ti-Ag alloys and applied mandible blocks. Conclusions The results of this study suggest that Ti-Ag alloys can be used to produce miniplates with high mechanical properties, as well as considerable biocompatibility, osteosynthesis ability, and Ag ion–release properties. Further studies, including preclinical investigations, are required to enable clinical use of Ti-Ag bone plates.

AB - Purpose Titanium (Ti) alloys have received considerable attention as materials for oral and maxillofacial surgery, which require high mechanical strength, osteosynthesis, and biocompatibility. The objective was to implant miniplates fabricated from commercially pure Ti (CP Ti) and newly developed Ti–silver (Ag) alloy in fractured mandibles of adult dogs after preliminary mechanical and biological characterization. Materials and Methods The surface characteristics, biocompatibility, and pre-osteoblast adhesion and proliferation of CP Ti (grade 3) and Ti-Ag (2 at% Ag) alloys were evaluated. Next, the bending strength of 6- and 8-hole miniplates fabricated from CP Ti and Ti-Ag was compared according to ISO (International Organization for Standardization) 9585. Six-hole miniplates were implanted for 12 weeks in fractured mandibles of adult dogs. The Ag ion concentration in each alloy and implanted bone block with soft tissue was measured by inductively coupled plasma mass spectroscopy after euthanasia according to ISO 10993-12. Results Precipitated Ag was detected in Ti-Ag by alpha- and beta-phase Ti in x-ray powder diffraction. The biocompatibility with pre-osteoblasts of Ti-Ag and CP Ti was comparable in terms of cytotoxicity, cell adhesion, and proliferation (P > .05). Ti-Ag miniplates had up to 3-fold greater bending strength than CP Ti miniplates (P < .05). An in vivo study showed that CP Ti and Ti-Ag miniplates had comparable soft and hard tissue regeneration ability (P > .05). Ag ions were detected in Ti-Ag alloys and applied mandible blocks. Conclusions The results of this study suggest that Ti-Ag alloys can be used to produce miniplates with high mechanical properties, as well as considerable biocompatibility, osteosynthesis ability, and Ag ion–release properties. Further studies, including preclinical investigations, are required to enable clinical use of Ti-Ag bone plates.

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