Tobacco GTBP1, a homolog of human heterogeneous nuclear ribonucleoprotein, protects telomeres from aberrant homologous recombination

Yong Woo Lee, Woo Taek Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Telomeres are nucleoprotein complexes essential for the integrity of eukaryotic chromosomes. Cellular roles of singlestrandedtelomeric DNA binding proteins have been extensively described in yeast and animals, but our knowledge about plant single-strand telomeric factors is rudimentary. Here, we investigated Nicotiana tabacum G-strand-specific singlestranded telomere binding proteins (GTBPs), homologs of a human heterogeneous nuclear ribonucleoprotein. GTBPs bound specifically to the plant single-stranded (TTTAGGG) 4 telomeric repeat element in vitro and were associated with telomeric sequences in tobacco BY-2 suspension cells. Transgenic plants (35S:RNAi-GTBP1), in which GTBP1 was suppressed, exhibited severe developmental anomalies. In addition, the chromosomes of 35S:RNAi-GTBP1 cells displayed elongated telomeres, frequent formation of extrachromosomal telomeric circles, and numerous abnormal anaphase bridges, indicating that GTBP1 knockdown tobacco plants experienced genome instability. GTBP1 inhibited strand invasion, an initial step in interchromosomal homologous recombination. We propose that GTBP1 plays a critical role in telomere structure and function by preventing aberrant interchromosomal telomeric homologous recombination in tobacco.

Original languageEnglish
Pages (from-to)2781-2795
Number of pages15
JournalPlant Cell
Volume22
Issue number8
DOIs
Publication statusPublished - 2010 Aug 1

Fingerprint

Heterogeneous-Nuclear Ribonucleoproteins
ribonucleoproteins
Homologous Recombination
homologous recombination
telomeres
Telomere
Tobacco
tobacco
Telomere-Binding Proteins
RNA Interference
Chromosomes
binding proteins
Plant Genome
Anaphase
Nucleoproteins
Genomic Instability
Genetically Modified Plants
DNA-Binding Proteins
chromosome addition
nucleoproteins

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

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abstract = "Telomeres are nucleoprotein complexes essential for the integrity of eukaryotic chromosomes. Cellular roles of singlestrandedtelomeric DNA binding proteins have been extensively described in yeast and animals, but our knowledge about plant single-strand telomeric factors is rudimentary. Here, we investigated Nicotiana tabacum G-strand-specific singlestranded telomere binding proteins (GTBPs), homologs of a human heterogeneous nuclear ribonucleoprotein. GTBPs bound specifically to the plant single-stranded (TTTAGGG) 4 telomeric repeat element in vitro and were associated with telomeric sequences in tobacco BY-2 suspension cells. Transgenic plants (35S:RNAi-GTBP1), in which GTBP1 was suppressed, exhibited severe developmental anomalies. In addition, the chromosomes of 35S:RNAi-GTBP1 cells displayed elongated telomeres, frequent formation of extrachromosomal telomeric circles, and numerous abnormal anaphase bridges, indicating that GTBP1 knockdown tobacco plants experienced genome instability. GTBP1 inhibited strand invasion, an initial step in interchromosomal homologous recombination. We propose that GTBP1 plays a critical role in telomere structure and function by preventing aberrant interchromosomal telomeric homologous recombination in tobacco.",
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Tobacco GTBP1, a homolog of human heterogeneous nuclear ribonucleoprotein, protects telomeres from aberrant homologous recombination. / Lee, Yong Woo; Kim, Woo Taek.

In: Plant Cell, Vol. 22, No. 8, 01.08.2010, p. 2781-2795.

Research output: Contribution to journalArticle

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