Toll-like Receptor Expression in Patients With Renal Allograft Dysfunction

J. Kwon, J. Park, D. Lee, Y. S. Kim, H. J. Jeong

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Toll-like receptor (TLR) is known to be a mediator of innate immunity, but recent reports have shown that TLR provides a link to adaptive immunity involved in allograft rejection. To explore the expression patterns in various conditions of renal transplantation, we examined TLR subunit mRNA expressions in renal allograft biopsies of acute rejection (AR; n = 11), chronic rejection (CR; n = 15), chronic cyclosporine nephrotoxicity (CsAN; n = 22), and immunoglobulin A nephropathy (IgAN; n = 9) patients. Control tissues (n = 7) were obtained from normal renal cortical tissue of renal cell carcinoma patients. The diagnosis was made according to the Banff 97 classification. The expressions of TLR 2, 3, 4, and 9 mRNA were analyzed by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) using SYBR green. Statistical analysis was performed using analysis of variance (ANOVA) and the Student t test. TLR 2 and 3 mRNA expressions were not significantly different in any group (P > .05). In contrast, TLR 4 mRNA expression was significantly increased in all allograft groups compared with that of controls, and significantly higher in the CsAN than other transplant groups (P < .05). TLR 9 mRNA expression was up-regulated in CsAN and IgAN compared with AR and CR (P < .05). These results suggested that TLR4 mRNA expression was increased in renal allograft patients with chronic allograft dysfunction. Further studies are needed to correlate TLR subtypes with various causes of graft dysfunction among renal allograft patients.

Original languageEnglish
Pages (from-to)3479-3480
Number of pages2
JournalTransplantation Proceedings
Volume40
Issue number10
DOIs
Publication statusPublished - 2008 Dec 1

Fingerprint

Toll-Like Receptors
Allografts
Kidney
Messenger RNA
Toll-Like Receptor 3
Toll-Like Receptor 2
IGA Glomerulonephritis
Toll-Like Receptor 9
Transplants
Toll-Like Receptor 4
Adaptive Immunity
Reverse Transcriptase Polymerase Chain Reaction
Renal Cell Carcinoma
Innate Immunity
Kidney Transplantation
Cyclosporine
Real-Time Polymerase Chain Reaction
Analysis of Variance
Students
Biopsy

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Kwon, J. ; Park, J. ; Lee, D. ; Kim, Y. S. ; Jeong, H. J. / Toll-like Receptor Expression in Patients With Renal Allograft Dysfunction. In: Transplantation Proceedings. 2008 ; Vol. 40, No. 10. pp. 3479-3480.
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Toll-like Receptor Expression in Patients With Renal Allograft Dysfunction. / Kwon, J.; Park, J.; Lee, D.; Kim, Y. S.; Jeong, H. J.

In: Transplantation Proceedings, Vol. 40, No. 10, 01.12.2008, p. 3479-3480.

Research output: Contribution to journalArticle

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