Tom40 protein import channel binds to non-native proteins and prevents their aggregation

Masatoshi Esaki, Takashi Kanamori, Shuh Ichi Nishikawa, Injae Shin, Peter G. Schultz, Toshiya Endo

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Mitochondria contain the translocator of the outer mitochondrial membrane (TOM) for protein entry into the organelle, and its subunit Tom40 forms a protein-conducting channel. Here we report the role of Tom40 in protein translocation across the membrane. The site-specific photocrosslinking experiment revealed that translocating unfolded or loosely folded precursor segments of up to 90 residues can be associated with Tom40. Purified Tom40 bound to non-native proteins and suppressed their aggregation when they are prone to aggregate. A denatured protein bound to the Tom40 channel blocked the protein import into mitochondria. These results indicate that, in contrast to the nonstick tunnel of the ribosome for polypeptide exit, the Tom40 channel offers an optimized environment to translocating non-native precursor proteins by preventing their aggregation.

Original languageEnglish
Pages (from-to)988-994
Number of pages7
JournalNature Structural Biology
Volume10
Issue number12
DOIs
Publication statusPublished - 2003 Dec 1

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Agglomeration
Mitochondria
Proteins
Protein Precursors
Mitochondrial Proteins
Mitochondrial Membranes
Protein Transport
Ribosomes
Organelles
Membrane Proteins
Tunnels
Peptides
Membranes
Experiments

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Genetics

Cite this

Esaki, Masatoshi ; Kanamori, Takashi ; Nishikawa, Shuh Ichi ; Shin, Injae ; Schultz, Peter G. ; Endo, Toshiya. / Tom40 protein import channel binds to non-native proteins and prevents their aggregation. In: Nature Structural Biology. 2003 ; Vol. 10, No. 12. pp. 988-994.
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Esaki, M, Kanamori, T, Nishikawa, SI, Shin, I, Schultz, PG & Endo, T 2003, 'Tom40 protein import channel binds to non-native proteins and prevents their aggregation', Nature Structural Biology, vol. 10, no. 12, pp. 988-994. https://doi.org/10.1038/nsb1008

Tom40 protein import channel binds to non-native proteins and prevents their aggregation. / Esaki, Masatoshi; Kanamori, Takashi; Nishikawa, Shuh Ichi; Shin, Injae; Schultz, Peter G.; Endo, Toshiya.

In: Nature Structural Biology, Vol. 10, No. 12, 01.12.2003, p. 988-994.

Research output: Contribution to journalArticle

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