Tools for probing local circuits: High-density silicon probes combined with optogenetics

György Buzsáki, Eran Stark, Antal Berényi, Dion Khodagholy, Daryl R. Kipke, Euisik Yoon, Kensall D. Wise

Research output: Contribution to journalReview article

135 Citations (Scopus)


To understand how function arises from the interactions between neurons, it is necessary to use methods that allow the monitoring of brain activity at the single-neuron, single-spike level and the targeted manipulation of the diverse neuron types selectively in a closed-loop manner. Large-scale recordings of neuronal spiking combined with optogenetic perturbation of identified individual neurons has emerged as a suitable method for such tasks in behaving animals. To fully exploit the potential power of these methods, multiple steps of technical innovation are needed. We highlight the current state of the art in electrophysiological recording methods, combined with optogenetics, and discuss directions for progress. In addition, we point to areas where rapid development is in progress and discuss topics where near-term improvements are possible and needed.

Original languageEnglish
Pages (from-to)92-105
Number of pages14
Issue number1
Publication statusPublished - 2015 Apr 8


All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Buzsáki, G., Stark, E., Berényi, A., Khodagholy, D., Kipke, D. R., Yoon, E., & Wise, K. D. (2015). Tools for probing local circuits: High-density silicon probes combined with optogenetics. Neuron, 86(1), 92-105.