Top-down lipidomic analysis of human lipoproteins by chip-type asymmetrical flow field-flow fractionation-electrospray ionization-tandem mass spectrometry

Ki Hun Kim, Ju Yong Lee, Sangsoo Lim, Myeong Hee Moon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This study demonstrates the potential utility of on-line chip-type asymmetrical flow field-flow fractionation (cAF4) and electrospray ionization tandem mass spectrometry (ESI-MS-MS) for the top-down lipidomic analysis of human lipoproteins. Utilizing a cAF4, which is a miniaturized AF4 channel operated with a micro flow rate regime, enabled high density lipoprotein (HDL) and low density lipoprotein (LDL) to be separated by hydrodynamic diameter in an aqueous solution with the simultaneous desalting of lipoproteins. On-line desalting was found to enhance the ionization of lipoproteinic lipid molecules during the feeding of cAF4 effluent to ESI-MS when compared to the direct infusion of lipoproteins to MS. An evaluation of top-down lipidomic analysis was performed to test the efficiency of in-source fragmentation during cAF4-ESI-MS in the dissociation of lipoprotein particles into individual lipid molecules. This study demonstrates the structural identification of the following lipid classes: phosphatidylcholines (PCs), cholesteryl esters (CEs), and regioisomers of triacylglycerols (TAGs) having an identical mass but different acyl chains and dimeric forms of TAGs in the positive ion mode, and phosphatidylglycerols (PGs), phosphatidic acids (PAs), phosphatidyinositols (PIs), and their lyso species in the negative ion mode. The developed method was applied to plasma samples from patients with coronary artery disease (CAD) for the separation of HDL and LDL and for the simultaneous analysis of lipoproteinic lipids, resulting in the identification of 11 PCs, 9 PGs, 4 PAs, 2 PIs, 2 PEs, 18 TAGs, and 6 CEs.

Original languageEnglish
Pages (from-to)92-97
Number of pages6
JournalJournal of Chromatography A
Volume1280
DOIs
Publication statusPublished - 2013 Mar 8

Fingerprint

Field Flow Fractionation
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Fractionation
Tandem Mass Spectrometry
Lipoproteins
Mass spectrometry
Flow fields
Lipids
Salt removal
Phosphatidylglycerols
Phosphatidic Acids
Triglycerides
Cholesterol Esters
HDL Lipoproteins
Phosphatidylcholines
LDL Lipoproteins
Ions
Molecules
Hydrodynamics

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

@article{d4440f4fe9514371861dc85c5d3ee7d0,
title = "Top-down lipidomic analysis of human lipoproteins by chip-type asymmetrical flow field-flow fractionation-electrospray ionization-tandem mass spectrometry",
abstract = "This study demonstrates the potential utility of on-line chip-type asymmetrical flow field-flow fractionation (cAF4) and electrospray ionization tandem mass spectrometry (ESI-MS-MS) for the top-down lipidomic analysis of human lipoproteins. Utilizing a cAF4, which is a miniaturized AF4 channel operated with a micro flow rate regime, enabled high density lipoprotein (HDL) and low density lipoprotein (LDL) to be separated by hydrodynamic diameter in an aqueous solution with the simultaneous desalting of lipoproteins. On-line desalting was found to enhance the ionization of lipoproteinic lipid molecules during the feeding of cAF4 effluent to ESI-MS when compared to the direct infusion of lipoproteins to MS. An evaluation of top-down lipidomic analysis was performed to test the efficiency of in-source fragmentation during cAF4-ESI-MS in the dissociation of lipoprotein particles into individual lipid molecules. This study demonstrates the structural identification of the following lipid classes: phosphatidylcholines (PCs), cholesteryl esters (CEs), and regioisomers of triacylglycerols (TAGs) having an identical mass but different acyl chains and dimeric forms of TAGs in the positive ion mode, and phosphatidylglycerols (PGs), phosphatidic acids (PAs), phosphatidyinositols (PIs), and their lyso species in the negative ion mode. The developed method was applied to plasma samples from patients with coronary artery disease (CAD) for the separation of HDL and LDL and for the simultaneous analysis of lipoproteinic lipids, resulting in the identification of 11 PCs, 9 PGs, 4 PAs, 2 PIs, 2 PEs, 18 TAGs, and 6 CEs.",
author = "Kim, {Ki Hun} and Lee, {Ju Yong} and Sangsoo Lim and Moon, {Myeong Hee}",
year = "2013",
month = "3",
day = "8",
doi = "10.1016/j.chroma.2013.01.025",
language = "English",
volume = "1280",
pages = "92--97",
journal = "Journal of Chromatography A",
issn = "0021-9673",

}

Top-down lipidomic analysis of human lipoproteins by chip-type asymmetrical flow field-flow fractionation-electrospray ionization-tandem mass spectrometry. / Kim, Ki Hun; Lee, Ju Yong; Lim, Sangsoo; Moon, Myeong Hee.

In: Journal of Chromatography A, Vol. 1280, 08.03.2013, p. 92-97.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Top-down lipidomic analysis of human lipoproteins by chip-type asymmetrical flow field-flow fractionation-electrospray ionization-tandem mass spectrometry

AU - Kim, Ki Hun

AU - Lee, Ju Yong

AU - Lim, Sangsoo

AU - Moon, Myeong Hee

PY - 2013/3/8

Y1 - 2013/3/8

N2 - This study demonstrates the potential utility of on-line chip-type asymmetrical flow field-flow fractionation (cAF4) and electrospray ionization tandem mass spectrometry (ESI-MS-MS) for the top-down lipidomic analysis of human lipoproteins. Utilizing a cAF4, which is a miniaturized AF4 channel operated with a micro flow rate regime, enabled high density lipoprotein (HDL) and low density lipoprotein (LDL) to be separated by hydrodynamic diameter in an aqueous solution with the simultaneous desalting of lipoproteins. On-line desalting was found to enhance the ionization of lipoproteinic lipid molecules during the feeding of cAF4 effluent to ESI-MS when compared to the direct infusion of lipoproteins to MS. An evaluation of top-down lipidomic analysis was performed to test the efficiency of in-source fragmentation during cAF4-ESI-MS in the dissociation of lipoprotein particles into individual lipid molecules. This study demonstrates the structural identification of the following lipid classes: phosphatidylcholines (PCs), cholesteryl esters (CEs), and regioisomers of triacylglycerols (TAGs) having an identical mass but different acyl chains and dimeric forms of TAGs in the positive ion mode, and phosphatidylglycerols (PGs), phosphatidic acids (PAs), phosphatidyinositols (PIs), and their lyso species in the negative ion mode. The developed method was applied to plasma samples from patients with coronary artery disease (CAD) for the separation of HDL and LDL and for the simultaneous analysis of lipoproteinic lipids, resulting in the identification of 11 PCs, 9 PGs, 4 PAs, 2 PIs, 2 PEs, 18 TAGs, and 6 CEs.

AB - This study demonstrates the potential utility of on-line chip-type asymmetrical flow field-flow fractionation (cAF4) and electrospray ionization tandem mass spectrometry (ESI-MS-MS) for the top-down lipidomic analysis of human lipoproteins. Utilizing a cAF4, which is a miniaturized AF4 channel operated with a micro flow rate regime, enabled high density lipoprotein (HDL) and low density lipoprotein (LDL) to be separated by hydrodynamic diameter in an aqueous solution with the simultaneous desalting of lipoproteins. On-line desalting was found to enhance the ionization of lipoproteinic lipid molecules during the feeding of cAF4 effluent to ESI-MS when compared to the direct infusion of lipoproteins to MS. An evaluation of top-down lipidomic analysis was performed to test the efficiency of in-source fragmentation during cAF4-ESI-MS in the dissociation of lipoprotein particles into individual lipid molecules. This study demonstrates the structural identification of the following lipid classes: phosphatidylcholines (PCs), cholesteryl esters (CEs), and regioisomers of triacylglycerols (TAGs) having an identical mass but different acyl chains and dimeric forms of TAGs in the positive ion mode, and phosphatidylglycerols (PGs), phosphatidic acids (PAs), phosphatidyinositols (PIs), and their lyso species in the negative ion mode. The developed method was applied to plasma samples from patients with coronary artery disease (CAD) for the separation of HDL and LDL and for the simultaneous analysis of lipoproteinic lipids, resulting in the identification of 11 PCs, 9 PGs, 4 PAs, 2 PIs, 2 PEs, 18 TAGs, and 6 CEs.

UR - http://www.scopus.com/inward/record.url?scp=84873525917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873525917&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2013.01.025

DO - 10.1016/j.chroma.2013.01.025

M3 - Article

C2 - 23375771

AN - SCOPUS:84873525917

VL - 1280

SP - 92

EP - 97

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

ER -