Topoisomerase II-mediated DNA cleavage on the cruciform structure formed within the 5′ Upstream region of the human β-globin gene

Gun Eui Lee, Jun Hyun Kim, In Kwon Chung

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A 52 base pair alternating purine-pyrimidine (RY) repeat sequence lies in the 5′ upstream region of the human β-globin gene. The structural transition of a plasmid containing this repeat was analyzed by two-dimensional gel electrophoresis. These conformational studies indicate that the 52 bp RY repeat undergoes local transition from the right-handed B-DNA into a cruciform DNA under torsional stress and the transition initiates at a threshold level of negative supercoiling (-σ = 0.042). The superhelicity-dependent S1 nuclease cleavage sites were mapped only within the RY repeat and no nicking was observed outside of the repeat. In view of the fact that DNA topoisomerase II is highly reactive towards RY repeat which can adopt unusual DNA conformation, we have investigated the effects of the superhelicity-dependent conformational transition of the 52 bp RY repeat on topoisomerase II cleavages. Cleavage reactions were performed on the pRYG plasmid with varying levels of negative superhelical densities ranging from O to -0.074. Under the low torsional stress, topoisomerase II cleavage activity at the RY repeat gradually increased with the increasing levels of negative superhelical densities. However, over a threshold level of negative supercoiling for cruciform conformation, the intensities of enzyme cleavage sites at the RY repeat were essentially identical. These results suggest that topoisomerase II can bind and cleave the cruciform structure in a dynamic process identical to duplex B-DNA.

Original languageEnglish
Pages (from-to)424-430
Number of pages7
JournalMolecules and cells
Volume8
Issue number4
Publication statusPublished - 1998 Aug 31

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Topoisomerase II-mediated DNA cleavage on the cruciform structure formed within the 5′ Upstream region of the human β-globin gene'. Together they form a unique fingerprint.

Cite this