Towards a proteomic analysis of atopic dermatitis: A two-dimensional- polyacrylamide gel electrophoresis/mass spectrometric analysis of cultured patient-derived fibroblasts

Yong Doo Park, So Yeon Kim, Hee Sun Jang, Eun Young Seo, Jung Hyun Namkung, Kyung Seok Park, Yun Cho Sang, Young Ki Paik, Jun Mo Yang

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease typically characterized by a distribution of eczematous skin lesions with lichenification, pruritic excoriations, and dry skin with wide varieties of pathophysiologic aspects. Recently, AD was divided into extrinsic and intrinsic forms according to the presence or absence of an allergy. We investigated alterations in protein expression in primary cultured AD cells from the patient biopsy samples by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight. In the primary cultured fibroblasts, we obtained 31 candidate proteins from the two-dimensional gel image analysis in which 18 proteins were up-regulated, eight proteins were down-regulated and five proteins were post-translationally modified. From these 2-DE results, we found several candidate genes matched proteomic expression patterns by semi-quantitative reverse transcription PCR. Since the exact mechanism of atopic alterations in fibroblasts remains unknown, our results may provide new clues to aid in understanding AD.

Original languageEnglish
Pages (from-to)3446-3455
Number of pages10
JournalProteomics
Volume4
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1

Fingerprint

Electrophoresis, Gel, Two-Dimensional
Atopic Dermatitis
Fibroblasts
Electrophoresis
Proteomics
Skin
Proteins
Gels
Allergies
Biopsy
Transcription
Skin Diseases
Image analysis
Reverse Transcription
Ionization
Desorption
Hypersensitivity
Lasers
Genes
polyacrylamide gels

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Park, Yong Doo ; Kim, So Yeon ; Jang, Hee Sun ; Seo, Eun Young ; Namkung, Jung Hyun ; Park, Kyung Seok ; Sang, Yun Cho ; Paik, Young Ki ; Yang, Jun Mo. / Towards a proteomic analysis of atopic dermatitis : A two-dimensional- polyacrylamide gel electrophoresis/mass spectrometric analysis of cultured patient-derived fibroblasts. In: Proteomics. 2004 ; Vol. 4, No. 11. pp. 3446-3455.
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Towards a proteomic analysis of atopic dermatitis : A two-dimensional- polyacrylamide gel electrophoresis/mass spectrometric analysis of cultured patient-derived fibroblasts. / Park, Yong Doo; Kim, So Yeon; Jang, Hee Sun; Seo, Eun Young; Namkung, Jung Hyun; Park, Kyung Seok; Sang, Yun Cho; Paik, Young Ki; Yang, Jun Mo.

In: Proteomics, Vol. 4, No. 11, 01.11.2004, p. 3446-3455.

Research output: Contribution to journalArticle

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AU - Namkung, Jung Hyun

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AU - Paik, Young Ki

AU - Yang, Jun Mo

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