Towards an immunodiagnostic test for leprosy

Romulo Aráoz, Nadine Honoré, Sayera Banu, Caroline Demangel, Yakouba Cissoko, Charles Arama, Mohammad Khaja Mafij Uddin, S. K. Abdul Hadi, Marc Monot, Sang Nae Cho, Baohong Ji, Patrick J. Brennan, Samba Sow, Stewart T. Cole

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38 Citations (Scopus)

Abstract

In addition to multidrug therapy, elimination of leprosy requires improved diagnostic methods. Using a comparative genomics approach, 17 potential protein antigens (MLP) that are restricted to Mycobacterium leprae, or of limited distribution, were produced and tested for antigen-specific immune responses on leprosy patients, healthy contacts of leprosy patients, and tuberculosis patients in Mali and Bangladesh, as well as on non-endemic controls. T-cell antigenicity of MLP was confirmed by IFN-γ production in whole-blood assays with the highest responses observed in paucibacillary leprosy patients and healthy contacts. Four MLP behaved well in both countries and induced significantly different responses between the study groups. Peptides carrying T cell epitopes from one of the antigens gave promising results in restimulation assays in mice and immune responses were not influenced by prior exposure to BCG or environmental mycobacteria. This study provides the immunological framework for the development of a specific, peptide-based immunodiagnostic test for leprosy.

Original languageEnglish
Pages (from-to)2270-2276
Number of pages7
JournalMicrobes and Infection
Volume8
Issue number8
DOIs
Publication statusPublished - 2006 Jul 1

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Infectious Diseases

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  • Cite this

    Aráoz, R., Honoré, N., Banu, S., Demangel, C., Cissoko, Y., Arama, C., Mafij Uddin, M. K., Abdul Hadi, S. K., Monot, M., Cho, S. N., Ji, B., Brennan, P. J., Sow, S., & Cole, S. T. (2006). Towards an immunodiagnostic test for leprosy. Microbes and Infection, 8(8), 2270-2276. https://doi.org/10.1016/j.micinf.2006.04.002