Towards an immunodiagnostic test for leprosy

Romulo Aráoz; Nadine Honoré; Sayera Banu; Caroline Demangel; Yakouba Cissoko; Charles Arama; Mohammad Khaja Mafij Uddin; S. K. Abdul Hadi; Marc Monot; Sang Nae Cho; Baohong Ji; Patrick J. Brennan; Samba Sow; Stewart T. Cole

doi:10.1016/j.micinf.2006.04.002

Towards an immunodiagnostic test for leprosy

Romulo Aráoz, Nadine Honoré, Sayera Banu, Caroline Demangel, Yakouba Cissoko, Charles Arama, Mohammad Khaja Mafij Uddin, S. K. Abdul Hadi, Marc Monot, Sang Nae Cho, Baohong Ji, Patrick J. Brennan, Samba Sow, Stewart T. Cole

Department of Microbiology

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

In addition to multidrug therapy, elimination of leprosy requires improved diagnostic methods. Using a comparative genomics approach, 17 potential protein antigens (MLP) that are restricted to Mycobacterium leprae, or of limited distribution, were produced and tested for antigen-specific immune responses on leprosy patients, healthy contacts of leprosy patients, and tuberculosis patients in Mali and Bangladesh, as well as on non-endemic controls. T-cell antigenicity of MLP was confirmed by IFN-γ production in whole-blood assays with the highest responses observed in paucibacillary leprosy patients and healthy contacts. Four MLP behaved well in both countries and induced significantly different responses between the study groups. Peptides carrying T cell epitopes from one of the antigens gave promising results in restimulation assays in mice and immune responses were not influenced by prior exposure to BCG or environmental mycobacteria. This study provides the immunological framework for the development of a specific, peptide-based immunodiagnostic test for leprosy.

Original language	English
Pages (from-to)	2270-2276
Number of pages	7
Journal	Microbes and Infection
Volume	8
Issue number	8
DOIs	https://doi.org/10.1016/j.micinf.2006.04.002
Publication status	Published - 2006 Jul

Bibliographical note

Funding Information:
We thank all the patients and members of control groups for participating in this study and the staff of the various clinics for their support. This work was supported by the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR grant number A20527), the Association Française Raoul Follereau, and the National Institutes of Health, National Institute of Allergy and Infectious Diseases (grants no. 1 RO1-AI47197-01A1, NO1-AI25469).

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.micinf.2006.04.002

Cite this

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title = "Towards an immunodiagnostic test for leprosy",

abstract = "In addition to multidrug therapy, elimination of leprosy requires improved diagnostic methods. Using a comparative genomics approach, 17 potential protein antigens (MLP) that are restricted to Mycobacterium leprae, or of limited distribution, were produced and tested for antigen-specific immune responses on leprosy patients, healthy contacts of leprosy patients, and tuberculosis patients in Mali and Bangladesh, as well as on non-endemic controls. T-cell antigenicity of MLP was confirmed by IFN-γ production in whole-blood assays with the highest responses observed in paucibacillary leprosy patients and healthy contacts. Four MLP behaved well in both countries and induced significantly different responses between the study groups. Peptides carrying T cell epitopes from one of the antigens gave promising results in restimulation assays in mice and immune responses were not influenced by prior exposure to BCG or environmental mycobacteria. This study provides the immunological framework for the development of a specific, peptide-based immunodiagnostic test for leprosy.",

author = "Romulo Ar{\'a}oz and Nadine Honor{\'e} and Sayera Banu and Caroline Demangel and Yakouba Cissoko and Charles Arama and {Mafij Uddin}, {Mohammad Khaja} and {Abdul Hadi}, {S. K.} and Marc Monot and Cho, {Sang Nae} and Baohong Ji and Brennan, {Patrick J.} and Samba Sow and Cole, {Stewart T.}",

note = "Funding Information: We thank all the patients and members of control groups for participating in this study and the staff of the various clinics for their support. This work was supported by the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR grant number A20527), the Association Fran{\c c}aise Raoul Follereau, and the National Institutes of Health, National Institute of Allergy and Infectious Diseases (grants no. 1 RO1-AI47197-01A1, NO1-AI25469).",

year = "2006",

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doi = "10.1016/j.micinf.2006.04.002",

language = "English",

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AU - Aráoz, Romulo

AU - Honoré, Nadine

AU - Banu, Sayera

AU - Demangel, Caroline

AU - Cissoko, Yakouba

AU - Arama, Charles

AU - Mafij Uddin, Mohammad Khaja

AU - Abdul Hadi, S. K.

AU - Monot, Marc

AU - Cho, Sang Nae

AU - Ji, Baohong

AU - Brennan, Patrick J.

AU - Sow, Samba

AU - Cole, Stewart T.

N1 - Funding Information: We thank all the patients and members of control groups for participating in this study and the staff of the various clinics for their support. This work was supported by the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR grant number A20527), the Association Française Raoul Follereau, and the National Institutes of Health, National Institute of Allergy and Infectious Diseases (grants no. 1 RO1-AI47197-01A1, NO1-AI25469).

PY - 2006/7

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N2 - In addition to multidrug therapy, elimination of leprosy requires improved diagnostic methods. Using a comparative genomics approach, 17 potential protein antigens (MLP) that are restricted to Mycobacterium leprae, or of limited distribution, were produced and tested for antigen-specific immune responses on leprosy patients, healthy contacts of leprosy patients, and tuberculosis patients in Mali and Bangladesh, as well as on non-endemic controls. T-cell antigenicity of MLP was confirmed by IFN-γ production in whole-blood assays with the highest responses observed in paucibacillary leprosy patients and healthy contacts. Four MLP behaved well in both countries and induced significantly different responses between the study groups. Peptides carrying T cell epitopes from one of the antigens gave promising results in restimulation assays in mice and immune responses were not influenced by prior exposure to BCG or environmental mycobacteria. This study provides the immunological framework for the development of a specific, peptide-based immunodiagnostic test for leprosy.

AB - In addition to multidrug therapy, elimination of leprosy requires improved diagnostic methods. Using a comparative genomics approach, 17 potential protein antigens (MLP) that are restricted to Mycobacterium leprae, or of limited distribution, were produced and tested for antigen-specific immune responses on leprosy patients, healthy contacts of leprosy patients, and tuberculosis patients in Mali and Bangladesh, as well as on non-endemic controls. T-cell antigenicity of MLP was confirmed by IFN-γ production in whole-blood assays with the highest responses observed in paucibacillary leprosy patients and healthy contacts. Four MLP behaved well in both countries and induced significantly different responses between the study groups. Peptides carrying T cell epitopes from one of the antigens gave promising results in restimulation assays in mice and immune responses were not influenced by prior exposure to BCG or environmental mycobacteria. This study provides the immunological framework for the development of a specific, peptide-based immunodiagnostic test for leprosy.

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Towards an immunodiagnostic test for leprosy

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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