Towards establishment of a rice stress response interactome

Young Su Seo, Mawsheng Chern, Laura E. Bartley, Muho Han, Ki Hong Jung, Insuk Lee, Harkamal Walia, Todd Richter, Xia Xu, Peijian Cao, Wei Bai, Rajeshwari Ramanan, Fawn Amonpant, Loganathan Arul, Patrick E. Canlas, Randy Ruan, Chang Jin Park, Xuewei Chen, Sohyun Hwang, Jong Seong JeonPamela C. Ronald

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Rice (Oryza sativa) is a staple food for more than half the world and a model for studies of monocotyledonous species, which include cereal crops and candidate bioenergy grasses. A major limitation of crop production is imposed by a suite of abiotic and biotic stresses resulting in 30%-60% yield losses globally each year. To elucidate stress response signaling networks, we constructed an interactome of 100 proteins by yeast two-hybrid (Y2H) assays around key regulators of the rice biotic and abiotic stress responses. We validated the interactome using protein-protein interaction (PPI) assays, co-expression of transcripts, and phenotypic analyses. Using this interactome-guided prediction and phenotype validation, we identified ten novel regulators of stress tolerance, including two from protein classes not previously known to function in stress responses. Several lines of evidence support cross-talk between biotic and abiotic stress responses. The combination of focused interactome and systems analyses described here represents significant progress toward elucidating the molecular basis of traits of agronomic importance.

Original languageEnglish
Article numbere1002020
JournalPLoS Genetics
Volume7
Issue number4
DOIs
Publication statusPublished - 2011 Apr

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

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  • Cite this

    Seo, Y. S., Chern, M., Bartley, L. E., Han, M., Jung, K. H., Lee, I., Walia, H., Richter, T., Xu, X., Cao, P., Bai, W., Ramanan, R., Amonpant, F., Arul, L., Canlas, P. E., Ruan, R., Park, C. J., Chen, X., Hwang, S., ... Ronald, P. C. (2011). Towards establishment of a rice stress response interactome. PLoS Genetics, 7(4), [e1002020]. https://doi.org/10.1371/journal.pgen.1002020