Towards gene therapy based on femtosecond optical transfection

M. Antkowiak, M. L. Torres-Mapa, J. McGinty, M. Chahine, L. Bugeon, A. Rose, A. Finn, S. Moleirinho, K. Okuse, M. Dallman, P. French, S. E. Harding, P. Reynolds, F. Gunn-Moore, K. Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Gene therapy poses a great promise in treatment and prevention of a variety of diseases. However, crucial to studying and the development of this therapeutic approach is a reliable and efficient technique of gene and drug delivery into primary cell types. These cells, freshly derived from an organ or tissue, mimic more closely the in vivo state and present more physiologically relevant information compared to cultured cell lines. However, primary cells are known to be difficult to transfect and are typically transfected using viral methods, which are not only questionable in the context of an in vivo application but rely on time consuming vector construction and may also result in cell de-differentiation and loss of functionality. At the same time, well established non-viral methods do not guarantee satisfactory efficiency and viability. Recently, optical laser mediated poration of cell membrane has received interest as a viable gene and drug delivery technique. It has been shown to deliver a variety of biomolecules and genes into cultured mammalian cells; however, its applicability to primary cells remains to be proven. We demonstrate how optical transfection can be an enabling technique in research areas, such as neuropathic pain, neurodegenerative diseases, heart failure and immune or inflammatory-related diseases. Several primary cell types are used in this study, namely cardiomyocytes, dendritic cells, and neurons. We present our recent progress in optimizing this technique's efficiency and post-treatment cell viability for these types of cells and discuss future directions towards in vivo applications.

Original languageEnglish
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care III
DOIs
Publication statusPublished - 2012
EventBiophotonics: Photonic Solutions for Better Health Care III - Brussels, Belgium
Duration: 2012 Apr 162012 Apr 19

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8427
ISSN (Print)1605-7422

Conference

ConferenceBiophotonics: Photonic Solutions for Better Health Care III
CountryBelgium
CityBrussels
Period12/4/1612/4/19

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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