Towards high-throughput automated targeted femtosecond laser-based transfection of adherent cells

MacIej Antkowiak, Maria Leilani Torres-Mapa, Frank Gunn-Moore, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Femtosecond laser induced cell membrane poration has proven to be an attractive alternative to the classical methods of drug and gene delivery. It is a selective, sterile, non-contact technique that offers a highly localized operation, low toxicity and consistent performance. However, its broader application still requires the development of robust, high-throughput and user-friendly systems. We present a system capable of unassisted enhanced targeted optoinjection and phototransfection of adherent mammalian cells with a femtosecond laser. We demonstrate the advantages of a dynamic diffractive optical element, namely a spatial light modulator (SLM) for precise three dimensional positioning of the beam. It enables the implementation of a "point-and-shoot" system in which using the software interface a user simply points at the cell and a predefined sequence of precisely positioned doses can be applied. We show that irradiation in three axial positions alleviates the problem of exact beam positioning on the cell membrane and doubles the number of viably optoinjected cells when compared with a single dose. The presented system enables untargeted raster scan irradiation which provides transfection of adherent cells at the throughput of 1 cell per second.

Original languageEnglish
Title of host publicationFrontiers in Ultrafast Optics
Subtitle of host publicationBiomedical, Scientific, and Industrial Applications XI
DOIs
Publication statusPublished - 2011 Apr 18
EventFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI - San Francisco, CA, United States
Duration: 2011 Jan 232011 Jan 26

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7925
ISSN (Print)0277-786X

Conference

ConferenceFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI
CountryUnited States
CitySan Francisco, CA
Period11/1/2311/1/26

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Antkowiak, M., Torres-Mapa, M. L., Gunn-Moore, F., & Dholakia, K. (2011). Towards high-throughput automated targeted femtosecond laser-based transfection of adherent cells. In Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI [79250B] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7925). https://doi.org/10.1117/12.874645