Traceable clonal culture and chemodrug assay of heterogeneous prostate carcinoma PC3 cells in microfluidic single cell array chips

Jaehoon Chung, Patrick N. Ingram, Tom Bersano-Begey, Euisik Yoon

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cancer heterogeneity has received considerable attention for its role in tumor initiation and progression, and its implication for diagnostics and therapeutics in the clinic. To facilitate a cellular heterogeneity study in a low cost and highly efficient manner, we present a microfluidic platform that allows traceable clonal culture and characterization. The platform captures single cells into a microwell array and cultures them for clonal expansion, subsequently allowing on-chip characterization of clonal phenotype and response against drug treatments. Using a heterogeneous prostate cancer model, the PC3 cell line, we verified our prototype, identifying three different sub-phenotypes and correlating their clonal drug responsiveness to cell phenotype.

Original languageEnglish
Article number056502
JournalBiomicrofluidics
Volume8
Issue number6
DOIs
Publication statusPublished - 2014 Nov 14

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All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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