TRAIL-overexpressing adipose tissue-derived mesenchymal stem cells efficiently inhibit tumor growth in an H460 xenograft model

Young U.N. Choi; Yongdae Yoon; Pil Young Jung; Soonjae Hwang; Ju E.U.N. Hong; Woo Seung Kim; Joon Hyung Sohn; Ki Jong Rhee; Keum Seok Bae; Young W.O.O. Eom

doi:10.21873/CGP.20281

TRAIL-overexpressing adipose tissue-derived mesenchymal stem cells efficiently inhibit tumor growth in an H460 xenograft model

Young U.N. Choi, Yongdae Yoon, Pil Young Jung, Soonjae Hwang, Ju E.U.N. Hong, Woo Seung Kim, Joon Hyung Sohn, Ki Jong Rhee, Keum Seok Bae, Young W.O.O. Eom

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Abstract

Background/Aim: Mesenchymal stem cell-based tumor therapy is still limited due to the insufficient secretion of effectors and discrepancies between their in vitro and in vivo efficacy. We investigated whether genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had inhibitory effects on H460 tumor growth both in vitro and in an H460 xenograft model. Materials and Methods: Genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were obtained from plasmid transfection with pCMV3-TRAIL and -interferon (IFN)-β (producing ASC-TRAIL and ASC-IFN-β, respectively). Death of H460 cells co-cultured with ASCs, ASC-TRAIL, and ASC-IFN-β or exposed to their conditioned medium was evaluated via apoptosis and cytotoxicity assays. In addition, in an H460 xenograft model (n=10 per group), the antitumor potential of.

Original language	English
Pages (from-to)	569-577
Number of pages	9
Journal	Cancer Genomics and Proteomics
Volume	18
Issue number	4
DOIs	https://doi.org/10.21873/CGP.20281
Publication status	Published - 2021 Jul

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI17C1365), and by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded the Ministry of Education, Republic of Korea (NRF-2017R1D1A1A02019212).

Publisher Copyright:
© 2021 International Institute of Anticancer Research. All rights reserved.

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Genetics
Cancer Research

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "TRAIL-overexpressing adipose tissue-derived mesenchymal stem cells efficiently inhibit tumor growth in an H460 xenograft model",

abstract = "Background/Aim: Mesenchymal stem cell-based tumor therapy is still limited due to the insufficient secretion of effectors and discrepancies between their in vitro and in vivo efficacy. We investigated whether genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had inhibitory effects on H460 tumor growth both in vitro and in an H460 xenograft model. Materials and Methods: Genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were obtained from plasmid transfection with pCMV3-TRAIL and -interferon (IFN)-β (producing ASC-TRAIL and ASC-IFN-β, respectively). Death of H460 cells co-cultured with ASCs, ASC-TRAIL, and ASC-IFN-β or exposed to their conditioned medium was evaluated via apoptosis and cytotoxicity assays. In addition, in an H460 xenograft model (n=10 per group), the antitumor potential of.",

author = "Choi, {Young U.N.} and Yongdae Yoon and Jung, {Pil Young} and Soonjae Hwang and Hong, {Ju E.U.N.} and Kim, {Woo Seung} and Sohn, {Joon Hyung} and Rhee, {Ki Jong} and Bae, {Keum Seok} and Eom, {Young W.O.O.}",

note = "Funding Information: This work was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI17C1365), and by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded the Ministry of Education, Republic of Korea (NRF-2017R1D1A1A02019212). Publisher Copyright: {\textcopyright} 2021 International Institute of Anticancer Research. All rights reserved.",

year = "2021",

month = jul,

doi = "10.21873/CGP.20281",

language = "English",

volume = "18",

pages = "569--577",

journal = "Cancer Genomics and Proteomics",

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publisher = "International Institute of Anticancer Research",

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T1 - TRAIL-overexpressing adipose tissue-derived mesenchymal stem cells efficiently inhibit tumor growth in an H460 xenograft model

AU - Choi, Young U.N.

AU - Yoon, Yongdae

AU - Jung, Pil Young

AU - Hwang, Soonjae

AU - Hong, Ju E.U.N.

AU - Kim, Woo Seung

AU - Sohn, Joon Hyung

AU - Rhee, Ki Jong

AU - Bae, Keum Seok

AU - Eom, Young W.O.O.

N1 - Funding Information: This work was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI17C1365), and by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded the Ministry of Education, Republic of Korea (NRF-2017R1D1A1A02019212). Publisher Copyright: © 2021 International Institute of Anticancer Research. All rights reserved.

PY - 2021/7

Y1 - 2021/7

N2 - Background/Aim: Mesenchymal stem cell-based tumor therapy is still limited due to the insufficient secretion of effectors and discrepancies between their in vitro and in vivo efficacy. We investigated whether genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had inhibitory effects on H460 tumor growth both in vitro and in an H460 xenograft model. Materials and Methods: Genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were obtained from plasmid transfection with pCMV3-TRAIL and -interferon (IFN)-β (producing ASC-TRAIL and ASC-IFN-β, respectively). Death of H460 cells co-cultured with ASCs, ASC-TRAIL, and ASC-IFN-β or exposed to their conditioned medium was evaluated via apoptosis and cytotoxicity assays. In addition, in an H460 xenograft model (n=10 per group), the antitumor potential of.

AB - Background/Aim: Mesenchymal stem cell-based tumor therapy is still limited due to the insufficient secretion of effectors and discrepancies between their in vitro and in vivo efficacy. We investigated whether genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had inhibitory effects on H460 tumor growth both in vitro and in an H460 xenograft model. Materials and Methods: Genetically engineered adipose tissue-derived mesenchymal stem cells (ASCs) overexpressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) were obtained from plasmid transfection with pCMV3-TRAIL and -interferon (IFN)-β (producing ASC-TRAIL and ASC-IFN-β, respectively). Death of H460 cells co-cultured with ASCs, ASC-TRAIL, and ASC-IFN-β or exposed to their conditioned medium was evaluated via apoptosis and cytotoxicity assays. In addition, in an H460 xenograft model (n=10 per group), the antitumor potential of.

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TRAIL-overexpressing adipose tissue-derived mesenchymal stem cells efficiently inhibit tumor growth in an H460 xenograft model

Abstract

Bibliographical note

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