Transcriptional control of the HERV-H LTR element of the GSDML gene in human tissues and cancer cells

H. S. Sin, J. W. Huh, D. S. Kim, D. W. Kang, D. S. Min, T. H. Kim, H. S. Ha, H. H. Kim, S. Y. Lee, H. S. Kim

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) have been reported to serve as alternative promoters in functional genes. The GSDML (gasdermin-like protein) gene located on human chromosome 17q21 has been found to be an oncogenomic recombination hotspot. Here, we identified the LTR element of HERV-H with reverse orientation as an alternative promoter of the GSDML gene and analyzed its expression pattern in human tissues and cancer cells. A reporter gene assay of the promoter activity of the LTR on the GSDML gene in human cancer cell lines (HCT-116 and HeLa) and a kidney cell line (Cos7) of African green monkey indicated that the LTR promoter with reverse orientation had stronger promoter activity than forward one. The transcripts of this LTR-derived promoter were widely distributed in various human tissues and cancer cells, whereas the transcripts of the cellular promoter were found only in stomach tissues and some cancer cells (HCT116, MCF7, U937, C-33A, and PC3). These findings suggest that the LTR element on the GSDML gene was integrated into the hominoid lineage and acquired the role of transcriptional regulation of human tissues and cancer cells.

Original languageEnglish
Pages (from-to)1985-1994
Number of pages10
JournalArchives of Virology
Volume151
Issue number10
DOIs
Publication statusPublished - 2006 Oct

All Science Journal Classification (ASJC) codes

  • Virology

Fingerprint Dive into the research topics of 'Transcriptional control of the HERV-H LTR element of the GSDML gene in human tissues and cancer cells'. Together they form a unique fingerprint.

  • Cite this

    Sin, H. S., Huh, J. W., Kim, D. S., Kang, D. W., Min, D. S., Kim, T. H., Ha, H. S., Kim, H. H., Lee, S. Y., & Kim, H. S. (2006). Transcriptional control of the HERV-H LTR element of the GSDML gene in human tissues and cancer cells. Archives of Virology, 151(10), 1985-1994. https://doi.org/10.1007/s00705-006-0764-5