Transcriptional regulation of GSDML gene by antisense-oriented HERV-H LTR element

J. W. Huh, D. S. Kim, D. W. Kang, H. S. Ha, K. Ahn, Y. N. Noh, D. S. Min, K. T. Chang, H. S. Kim

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Abstract

During the course of hominoid evolution, a new transcript variant of the GSDML (gasdermin-like protein) gene was formed by the integration of the antisense-oriented HERV-H (human endogenous retrovirus) LTR (long terminal repeat) element. To investigate regions that are critical for transcriptional regulation of the GSDML gene, we generated seven deletion mutants from a full-length clone (clone 1/630) that includes the HERV-H LTR sequence and compared their expression levels relative to the full-length parental clone using a transient transfection assay. In the transient transfection assay, deletion of the 5′ flanking region (cellular origin) of the HERV-H LTR sequence led to a 4.5-fold increase in expression compared to the full-length clone, while deletion of the U5 region showed a significant decrease in transcriptional activity. Deletion of the 3′ flanking region of the LTR sequence (clone 42/451) showed similar transcriptional activity to a clone missing the 5′ flanking region of cellular origin (clone 42/630). Taken together, these data indicate that the HERV-H LTR sequence (viral origin) positively regulates transcriptional activity of the GSDML gene and that the 5′ flanking region sequence (cellular origin) exerts negative transcriptional regulation.

Original languageEnglish
Pages (from-to)1201-1205
Number of pages5
JournalArchives of Virology
Volume153
Issue number6
DOIs
Publication statusPublished - 2008 Jun 1

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All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Huh, J. W., Kim, D. S., Kang, D. W., Ha, H. S., Ahn, K., Noh, Y. N., Min, D. S., Chang, K. T., & Kim, H. S. (2008). Transcriptional regulation of GSDML gene by antisense-oriented HERV-H LTR element. Archives of Virology, 153(6), 1201-1205. https://doi.org/10.1007/s00705-008-0105-y