Transcriptome Analysis and the Prognostic Role of NUDC in Diffuse and Intestinal Gastric Cancer

Sang Ho Jeong, Miyeong Park, Sun Yi Park, Jiho Park, Tae Han Kim, Young Joon Lee, Eun Jung Jung, Young Tae Ju, Chi Young Jeong, Ju Yeon Kim, Gyung Hyuck Ko, Minhye Kim, Ki Taek Nam, James R. Goldenring

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Introduction: There have been few studies about gene differences between patients with diffuse-type gastric cancer and those with intestinal-type gastric cancer. The aim of this study was to compare the transcriptomes of signet ring cell gastric cancer (worst prognosis in diffuse-type) and well-differentiated gastric cancer (best prognosis in intestinal-type); NUDC was identified, and its prognostic role was studied. Materials and Methods: We performed next-generation sequencing with 5 well-differentiated gastric cancers and 3 of signet ring cell gastric cancer surgical samples. We performed gene enrichment and functional annotation analysis using the Database for Annotation, Visualization and Integrated Discovery bioinformatics resources. Immunohistochemistry was used to validate NUDC expression. Results: Overall, 900 genes showed significantly higher expression, 644 genes showed lower expression in signet ring cell gastric cancer than in well-differentiated gastric cancers, and there was a large difference in adhesion, vascular development, and cell-to-cell junction components between the 2 subtypes. We performed variant analysis and found 52 variants and 30 cancer driver genes, including NUDC. We analyzed NUDC expression in gastric cancer tissue and its relationship with prognosis. Cox proportional hazard analysis identified T stage, N stage, and NUDC expression as independent risk factors for survival (P < 0.05). The overall survival of the NUDC-positive group was significantly higher (53.2 ± 0.92 months) than that of the NUDC-negative group (44.6 ± 3.7 months) (P = 0.001) in Kaplan-Meier survival analysis. Conclusion: We found 30 cancer driver gene candidates and found that the NUDC-positive group showed significantly better survival than the NUDC-negative group via variant analysis.

Original languageEnglish
JournalTechnology in Cancer Research and Treatment
Publication statusPublished - 2021

Bibliographical note

Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by Biomedical Research Institute Fund (GNUHBRIF-2016-0006) from the Gyeongsang National University Hospital, and a National Research Foundation of Korea (NRF) grant funded by the Republic of Korean government (2017R1D1A1B03034344).

Publisher Copyright:
© The Author(s) 2021.

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


Dive into the research topics of 'Transcriptome Analysis and the Prognostic Role of NUDC in Diffuse and Intestinal Gastric Cancer'. Together they form a unique fingerprint.

Cite this