Transcriptome analysis of IBET-151, a bet inhibitor alone and in combination with paclitaxel in gastric cancer cells

Sun Kyoung Kang, Hyun Joo Bae, Woo Sun Kwon, Jingmin Che, Tae Soo Kim, Hyun Cheol Chung, Sun Young Rha

Research output: Contribution to journalArticlepeer-review

Abstract

BET inhibitor, as an epigenetic regulator inhibitor, reduces the expression of oncogenes such as Myc and Bcl-2, which affects cancer growth and development. However, it has modest activity because of the narrow therapeutic index. Therefore, combination therapy is necessary to increase the anti-tumor effect. Paclitaxel, an anti-mitotic inhibitor, is used as second-line therapy for gastric cancer (GC) as a monotherapy or combination. In this study, we performed RNA sequencing of GC cells treated with iBET-151 and/or paclitaxel to iden-tify the differentially expressed genes associated with possible mechanisms of synergistic effect. We also performed Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses to determine the most enriched terms and pathways of upregulated and downregulated genes. We found 460 genes in which iBET-151 and pacli-taxel combination treatment changed more than single-treatment or no-treatment. Thus, additional functional studies are needed, but our results provide the first evidence of the synergistic effect between iBET-151 and paclitaxel in regulating the transcriptome of GC cells.

Original languageEnglish
Article numbere37
Pages (from-to)1-11
Number of pages11
JournalGenomics and Informatics
Volume18
Issue number4
DOIs
Publication statusPublished - 2020

Bibliographical note

Funding Information:
We thank all members of the CMRC lab for discussion. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2020R1A2B5B02001452).

Publisher Copyright:
© 2020, Korea Genome Organization.

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Health Informatics

Fingerprint

Dive into the research topics of 'Transcriptome analysis of IBET-151, a bet inhibitor alone and in combination with paclitaxel in gastric cancer cells'. Together they form a unique fingerprint.

Cite this