Transcutaneous implantation of valproic acid-encapsulated dissolving microneedles induces hair regrowth

Shayan Fakhraei Lahiji, Seol Hwa Seo, Suyong Kim, Manita Dangol, Jiyong Shim, Cheng Guo Li, Yonghao Ma, Chisong Lee, Geonwoo Kang, Huisuk Yang, Kang Yell Choi, Hyungil Jung

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The interest in alternative material systems and delivery methods for treatment of androgenetic alopecia has been increasing in the recent decades. Topical application of valproic acid (VPA), an FDA-approved anticonvulsant drug, has been shown to effectively stimulate hair follicle (HF) regrowth by upregulating Wnt/β-catenin, a key pathway involved in initiation of HF development. Moreover, a majority of studies have suggested that cutaneous wound re-epithelialization is capable of inducing HF through Wnt/β-catenin pathway. Here, we report fabrication and evaluation of a novel VPA-encapsulating dissolving microneedle (DMN-VPA) that creates minimally invasive dermal micro-wounds upon application, significantly improving the VPA delivery efficiency. DMN-VPA not only delivers encapsulated VPA with higher accuracy than topical application, it also stimulates wound re-epithelialization signals involved in HF regrowth. Through a series of in vivo studies, we show that micro-wounding-mediated implantation of DMN-VPA upregulates expression of Wnt/β-catenin pathway, alkaline phosphatase, proliferating cell nuclear antigen, loricrin and HF stem cell markers, including keratin 15, and CD34 more effectively than topical application.

Original languageEnglish
Pages (from-to)69-79
Number of pages11
JournalBiomaterials
Volume167
DOIs
Publication statusPublished - 2018 Jun

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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    Fakhraei Lahiji, S., Seo, S. H., Kim, S., Dangol, M., Shim, J., Li, C. G., Ma, Y., Lee, C., Kang, G., Yang, H., Choi, K. Y., & Jung, H. (2018). Transcutaneous implantation of valproic acid-encapsulated dissolving microneedles induces hair regrowth. Biomaterials, 167, 69-79. https://doi.org/10.1016/j.biomaterials.2018.03.019