Transfection of arginine decarboxylase gene increases the neuronal differentiation of neural progenitor cells

Kiran Kumar Bokara, Jae Hwan Kim, Jae Young Kim, Jongeun Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Growing evidence suggests that the clinical use of neural progenitor cells (NPCs) is hampered by heterogeneity, poor neuronal yield and low survival rate. Recently, we reported that retrovirus-delivered human arginine decarboxylase (hADC) genes improve cell survival against oxidative insult in murine NPCs in vitro. This study investigates whether the induced expression of hADC gene in mNPCs induces any significant change in the cell fate commitment. The evaluation of induced hADC gene function was assessed by knockdown of hADC gene using specific siRNA. The hADC gene delivery triggered higher expression of N-CAM, cell adhesion molecule and MAP-2, neuronal marker. However, the hADC gene knockdown showed downregulation of N-CAM and MAP-2 expression suggesting that hADC gene delivery favors cell fate commitment of mNPCs towards neuronal lineage. Neurite outgrowth was significantly longer in the hADC infected cells. The neurotrophic signal, BDNF aided in the neuronal commitment, differentiation, and maturation of hADC-mNPCs through PI3K and ERK1/2 activation. The induction of neuron-like differentiation is believed to be regulated by the expression of GSK-3β and Wnt/β-catenin signaling pathways. Our findings suggest that hADC gene delivery favors cell fate commitment of mNPCs towards neuronal lineage, bring new advances in the field of neurogenesis and cell therapy.

Original languageEnglish
Pages (from-to)256-265
Number of pages10
JournalStem Cell Research
Volume17
Issue number2
DOIs
Publication statusPublished - 2016 Sep 1

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Transfection
Stem Cells
Genes
arginine decarboxylase
Gene Knockdown Techniques
Glycogen Synthase Kinase 3
Catenins
Wnt Signaling Pathway
Brain-Derived Neurotrophic Factor
Neurogenesis
Cell Adhesion Molecules
Retroviridae
Cell- and Tissue-Based Therapy
Phosphatidylinositol 3-Kinases
Small Interfering RNA
Cell Survival
Down-Regulation
Neurons

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Bokara, Kiran Kumar ; Kim, Jae Hwan ; Kim, Jae Young ; Lee, Jongeun. / Transfection of arginine decarboxylase gene increases the neuronal differentiation of neural progenitor cells. In: Stem Cell Research. 2016 ; Vol. 17, No. 2. pp. 256-265.
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Transfection of arginine decarboxylase gene increases the neuronal differentiation of neural progenitor cells. / Bokara, Kiran Kumar; Kim, Jae Hwan; Kim, Jae Young; Lee, Jongeun.

In: Stem Cell Research, Vol. 17, No. 2, 01.09.2016, p. 256-265.

Research output: Contribution to journalArticle

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