Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice

Ai Guo Wang; Hyung Bae Moon; Young Ho Lee; Seong Lan Yu; Hyun Jung Kwon; Ying Hao Han; Wan Fang; Tae Hoon Lee; Kyung Lib Jang; Sang Keun Kim; Dae Yeul Yu; Dong Seok Lee

doi:10.1038/emm.2004.76

Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice

Ai Guo Wang, Hyung Bae Moon, Young Ho Lee, Seong Lan Yu, Hyun Jung Kwon, Ying Hao Han, Wan Fang, Tae Hoon Lee, Kyung Lib Jang, Sang Keun Kim, Dae Yeul Yu, Dong Seok Lee

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S116I, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S116I transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.

Original language	English
Pages (from-to)	588-593
Number of pages	6
Journal	Experimental and Molecular Medicine
Volume	36
Issue number	6
DOIs	https://doi.org/10.1038/emm.2004.76
Publication status	Published - 2004 Dec 31

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Clinical Biochemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/emm.2004.76

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title = "Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice",

abstract = "Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S116I, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S116I transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.",

author = "Wang, {Ai Guo} and Moon, {Hyung Bae} and Lee, {Young Ho} and Yu, {Seong Lan} and Kwon, {Hyun Jung} and Han, {Ying Hao} and Wan Fang and Lee, {Tae Hoon} and Jang, {Kyung Lib} and Kim, {Sang Keun} and Yu, {Dae Yeul} and Lee, {Dong Seok}",

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doi = "10.1038/emm.2004.76",

language = "English",

volume = "36",

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T1 - Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice

AU - Wang, Ai Guo

AU - Moon, Hyung Bae

AU - Lee, Young Ho

AU - Yu, Seong Lan

AU - Kwon, Hyun Jung

AU - Han, Ying Hao

AU - Fang, Wan

AU - Lee, Tae Hoon

AU - Jang, Kyung Lib

AU - Kim, Sang Keun

AU - Yu, Dae Yeul

AU - Lee, Dong Seok

PY - 2004/12/31

Y1 - 2004/12/31

N2 - Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S116I, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S116I transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.

AB - Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S116I, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S116I transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.

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Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice

Abstract

All Science Journal Classification (ASJC) codes

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