Transgenic expression of Korean type hepatitis C virus core protein and related mutants in mice

Ai Guo Wang, Hyung Bae Moon, Young Ho Lee, Seong Lan Yu, Hyun Jung Kwon, Ying Hao Han, Wan Fang, Tae Hoon Lee, Kyung Lib Jang, Sang Keun Kim, Dae Yeul Yu, Dong Seok Lee

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) is a major causative agent in liver disease. In order to investigate if Korean type HCV core protein and its related mutants, S99Q and S116I, are cytopathic to liver, three types of transgenic mice were established. The expression of transgenes was confirmed by HCV specific RT-PCR and Western immunoblotting. The livers of all wild type core and S116I transgenic lineages remained largely histologically normal. However, the livers of the S99Q transgenic mice showed significant high level of cell dysplasia associated with the transgene expression in hepatocytes largely located around the central veins by in situ hybridization analysis. In conclusion, the mutant HCV core protein at S99Q may contribute to the progress of HCV induced liver disease.

Original languageEnglish
Pages (from-to)588-593
Number of pages6
JournalExperimental and Molecular Medicine
Volume36
Issue number6
DOIs
Publication statusPublished - 2004 Dec 31

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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