Transient drug-tolerance and permanent drug-resistance rely on the trehalose-catalytic shift in Mycobacterium tuberculosis

Jae Jin Lee, Sun Kyung Lee, Naomi Song, Temitope O. Nathan, Benjamin M. Swarts, Seok Yong Eum, Sabine Ehrt, Sangnae Cho, Hyungjin Eoh

Research output: Contribution to journalArticle

Abstract

Stochastic formation of Mycobacterium tuberculosis (Mtb) persisters achieves a high level of antibiotic-tolerance and serves as a source of multidrug-resistant (MDR) mutations. As conventional treatment is not effective against infections by persisters and MDR-Mtb, novel therapeutics are needed. Several approaches were proposed to kill persisters by altering their metabolism, obviating the need to target active processes. Here, we adapted a biofilm culture to model Mtb persister-like bacilli (PLB) and demonstrated that PLB underwent trehalose metabolism remodeling. PLB use trehalose as an internal carbon to biosynthesize central carbon metabolism intermediates instead of cell surface glycolipids, thus maintaining levels of ATP and antioxidants. Similar changes were identified in Mtb following antibiotic-treatment, and MDR-Mtb as mechanisms to circumvent antibiotic effects. This suggests that trehalose metabolism is associated not only with transient drug-tolerance but also permanent drug-resistance, and serves as a source of adjunctive therapeutic options, potentiating antibiotic efficacy by interfering with adaptive strategies.

Original languageEnglish
Number of pages1
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019 Jul 2

Fingerprint

Drug Tolerance
tuberculosis
Trehalose
Mycobacterium tuberculosis
Drug Resistance
Metabolism
antibiotics
metabolism
drugs
Bacilli
Bacillus
Anti-Bacterial Agents
shift
Multidrug-Resistant Tuberculosis
Pharmaceutical Preparations
Carbon
Glycolipids
Biofilms
biofilms
adenosine triphosphate

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Lee, Jae Jin ; Lee, Sun Kyung ; Song, Naomi ; Nathan, Temitope O. ; Swarts, Benjamin M. ; Eum, Seok Yong ; Ehrt, Sabine ; Cho, Sangnae ; Eoh, Hyungjin. / Transient drug-tolerance and permanent drug-resistance rely on the trehalose-catalytic shift in Mycobacterium tuberculosis. In: Nature communications. 2019 ; Vol. 10, No. 1.
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Transient drug-tolerance and permanent drug-resistance rely on the trehalose-catalytic shift in Mycobacterium tuberculosis. / Lee, Jae Jin; Lee, Sun Kyung; Song, Naomi; Nathan, Temitope O.; Swarts, Benjamin M.; Eum, Seok Yong; Ehrt, Sabine; Cho, Sangnae; Eoh, Hyungjin.

In: Nature communications, Vol. 10, No. 1, 02.07.2019.

Research output: Contribution to journalArticle

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