Transient expression of phospholipase D1 in developing rat hippocampus

Do Sik Min, Jeong Sun Choi, Myung Hoon Chun, Jin Woong Chung, Mun Yong Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated the distribution of phospholipase D1 (PLD1) protein in the developing rat hippocampus using an affinity-purified peptide antibody against PLD1. Immunoreactivity for PLD1 was first seen in some scattered cells in the hippocampus at embryonic day 18. At postnatal day 1 (P1), many PLD1 immunoreactive cells were observed in the CA1 and CA3 sectors, subiculum and the hilus of the dentate gyrus. During the first postnatal week, there was an abrupt increase of immunoreactive neurons in the hippocampus, and their number and intensity peaked at P7. During the second postnatal week, there was an abrupt decrease in the number of immunoreactive hippocampal neurons. By P14, no significant labeling was found in the hippocampus. These results corresponded well with those from Western blot analysis, suggesting that PLD1 may regulate the developmental processes of hippocampal neurons.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalNeuroscience Letters
Volume310
Issue number2-3
DOIs
Publication statusPublished - 2001 Sep 14

Fingerprint

Hippocampus
Neurons
Dentate Gyrus
Western Blotting
phospholipase D1
Peptides
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Min, Do Sik ; Choi, Jeong Sun ; Chun, Myung Hoon ; Chung, Jin Woong ; Lee, Mun Yong. / Transient expression of phospholipase D1 in developing rat hippocampus. In: Neuroscience Letters. 2001 ; Vol. 310, No. 2-3. pp. 125-128.
@article{4578fc5e161f42f08c5df8bad127662a,
title = "Transient expression of phospholipase D1 in developing rat hippocampus",
abstract = "We investigated the distribution of phospholipase D1 (PLD1) protein in the developing rat hippocampus using an affinity-purified peptide antibody against PLD1. Immunoreactivity for PLD1 was first seen in some scattered cells in the hippocampus at embryonic day 18. At postnatal day 1 (P1), many PLD1 immunoreactive cells were observed in the CA1 and CA3 sectors, subiculum and the hilus of the dentate gyrus. During the first postnatal week, there was an abrupt increase of immunoreactive neurons in the hippocampus, and their number and intensity peaked at P7. During the second postnatal week, there was an abrupt decrease in the number of immunoreactive hippocampal neurons. By P14, no significant labeling was found in the hippocampus. These results corresponded well with those from Western blot analysis, suggesting that PLD1 may regulate the developmental processes of hippocampal neurons.",
author = "Min, {Do Sik} and Choi, {Jeong Sun} and Chun, {Myung Hoon} and Chung, {Jin Woong} and Lee, {Mun Yong}",
year = "2001",
month = "9",
day = "14",
doi = "10.1016/S0304-3940(01)02091-2",
language = "English",
volume = "310",
pages = "125--128",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

Transient expression of phospholipase D1 in developing rat hippocampus. / Min, Do Sik; Choi, Jeong Sun; Chun, Myung Hoon; Chung, Jin Woong; Lee, Mun Yong.

In: Neuroscience Letters, Vol. 310, No. 2-3, 14.09.2001, p. 125-128.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Transient expression of phospholipase D1 in developing rat hippocampus

AU - Min, Do Sik

AU - Choi, Jeong Sun

AU - Chun, Myung Hoon

AU - Chung, Jin Woong

AU - Lee, Mun Yong

PY - 2001/9/14

Y1 - 2001/9/14

N2 - We investigated the distribution of phospholipase D1 (PLD1) protein in the developing rat hippocampus using an affinity-purified peptide antibody against PLD1. Immunoreactivity for PLD1 was first seen in some scattered cells in the hippocampus at embryonic day 18. At postnatal day 1 (P1), many PLD1 immunoreactive cells were observed in the CA1 and CA3 sectors, subiculum and the hilus of the dentate gyrus. During the first postnatal week, there was an abrupt increase of immunoreactive neurons in the hippocampus, and their number and intensity peaked at P7. During the second postnatal week, there was an abrupt decrease in the number of immunoreactive hippocampal neurons. By P14, no significant labeling was found in the hippocampus. These results corresponded well with those from Western blot analysis, suggesting that PLD1 may regulate the developmental processes of hippocampal neurons.

AB - We investigated the distribution of phospholipase D1 (PLD1) protein in the developing rat hippocampus using an affinity-purified peptide antibody against PLD1. Immunoreactivity for PLD1 was first seen in some scattered cells in the hippocampus at embryonic day 18. At postnatal day 1 (P1), many PLD1 immunoreactive cells were observed in the CA1 and CA3 sectors, subiculum and the hilus of the dentate gyrus. During the first postnatal week, there was an abrupt increase of immunoreactive neurons in the hippocampus, and their number and intensity peaked at P7. During the second postnatal week, there was an abrupt decrease in the number of immunoreactive hippocampal neurons. By P14, no significant labeling was found in the hippocampus. These results corresponded well with those from Western blot analysis, suggesting that PLD1 may regulate the developmental processes of hippocampal neurons.

UR - http://www.scopus.com/inward/record.url?scp=0035860523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035860523&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(01)02091-2

DO - 10.1016/S0304-3940(01)02091-2

M3 - Article

C2 - 11585583

AN - SCOPUS:0035860523

VL - 310

SP - 125

EP - 128

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2-3

ER -