Transient telomere dysfunction induces chromosomal instability and promotes carcinogenesis

Yvonne Begus-Nahrmann, Daniel Hartmann, Johann Kraus, Parisa Eshraghi, Annika Scheffold, Melanie Grieb, Volker Rasche, Peter Schirmacher, Han Wong Lee, Hans A. Kestler, André Lechel, K. Lenhard Rudolph

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Telomere shortening limits the proliferative capacity of a cell, but perhaps surprisingly, shortening is also known to be associated with increased rates of tumor initiation. A current hypothesis suggests that telomere dysfunction increases tumor initiation by induction of chromosomal instability, but that initiated tumors need to reactivate telomerase for genome stabilization and tumor progression. This concept has not been tested in vivo, since appropriate mouse models were lacking. Here, we analyzed hepatocarcinogenesis in a mouse model of inducible telomere dysfunction on a telomerase-proficient background, in telomerase knockout mice with chronic telomere dysfunction (G3 mTerc -/-), and in WT mice with functional telomeres and telomerase. Transient or chronic telomere dysfunction enhanced the rates of chromosomal aberrations during hepatocarcinogenesis, but only telomerase-proficient mice exhibited significantly increased rates of macroscopic tumor formation in response to telomere dysfunction. In contrast, telomere dysfunction resulted in pronounced accumulation of DNA damage, cell-cycle arrest, and apoptosis in telomerase-deficient liver tumors. Together, these data provide in vivo evidence that transient telomere dysfunction during early or late stages of tumorigenesis promotes chromosomal instability and carcinogenesis in telomerase-proficient mice.

Original languageEnglish
Pages (from-to)2283-2288
Number of pages6
JournalJournal of Clinical Investigation
Volume122
Issue number6
DOIs
Publication statusPublished - 2012 Jun 1

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Chromosomal Instability
Telomere
Telomerase
Carcinogenesis
Neoplasms
Telomere Shortening
Cell Cycle Checkpoints
Knockout Mice
Chromosome Aberrations
DNA Damage
Genome
Apoptosis
Liver

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Begus-Nahrmann, Y., Hartmann, D., Kraus, J., Eshraghi, P., Scheffold, A., Grieb, M., ... Rudolph, K. L. (2012). Transient telomere dysfunction induces chromosomal instability and promotes carcinogenesis. Journal of Clinical Investigation, 122(6), 2283-2288. https://doi.org/10.1172/JCI61745
Begus-Nahrmann, Yvonne ; Hartmann, Daniel ; Kraus, Johann ; Eshraghi, Parisa ; Scheffold, Annika ; Grieb, Melanie ; Rasche, Volker ; Schirmacher, Peter ; Lee, Han Wong ; Kestler, Hans A. ; Lechel, André ; Rudolph, K. Lenhard. / Transient telomere dysfunction induces chromosomal instability and promotes carcinogenesis. In: Journal of Clinical Investigation. 2012 ; Vol. 122, No. 6. pp. 2283-2288.
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Begus-Nahrmann, Y, Hartmann, D, Kraus, J, Eshraghi, P, Scheffold, A, Grieb, M, Rasche, V, Schirmacher, P, Lee, HW, Kestler, HA, Lechel, A & Rudolph, KL 2012, 'Transient telomere dysfunction induces chromosomal instability and promotes carcinogenesis', Journal of Clinical Investigation, vol. 122, no. 6, pp. 2283-2288. https://doi.org/10.1172/JCI61745

Transient telomere dysfunction induces chromosomal instability and promotes carcinogenesis. / Begus-Nahrmann, Yvonne; Hartmann, Daniel; Kraus, Johann; Eshraghi, Parisa; Scheffold, Annika; Grieb, Melanie; Rasche, Volker; Schirmacher, Peter; Lee, Han Wong; Kestler, Hans A.; Lechel, André; Rudolph, K. Lenhard.

In: Journal of Clinical Investigation, Vol. 122, No. 6, 01.06.2012, p. 2283-2288.

Research output: Contribution to journalArticle

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Begus-Nahrmann Y, Hartmann D, Kraus J, Eshraghi P, Scheffold A, Grieb M et al. Transient telomere dysfunction induces chromosomal instability and promotes carcinogenesis. Journal of Clinical Investigation. 2012 Jun 1;122(6):2283-2288. https://doi.org/10.1172/JCI61745