Treatment of FGF-2 on stem cells from inflamed dental pulp tissue from human deciduous teeth

J. C. Kim, J. C. Park, S. H. Kim, G. I. Im, B. S. Kim, J. B. Lee, E. Y. Choi, JeSeon Song, K. S. Cho, ChangSung Kim

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: The purposes of this study were to isolate and characterize stem cells from inflamed pulp tissue of human functional deciduous teeth (iSHFD) and to evaluate the influence of fibroblastic growth factor-2 (FGF-2) on the regenerative potential. Materials and methods: We successfully isolated mesenchymal stem cells (MSCs) from the inflamed dental pulp tissue of human deciduous teeth and demonstrated that their regenerative potential could be enhanced by the application of FGF-2 (20 ng ml -1 ) during ex vivo expansion. Isolated stem cells expanded in FGF-2 were characterized using a colony-forming assay, proliferation, migration, in vitro differentiation, in vivo ectopic transplantation assay, and gene expression profiling. Results: MSCs isolated from the inflamed pulp tissue of functional deciduous teeth potentially possess the qualities of those from human exfoliated deciduous teeth. FGF-2 applied to iSHFD during expansion enhanced the colony-forming efficiency of these cells, increased their proliferation and migration potential, and reduced their differentiation potential in vitro. However, the ectopic transplantation of iSHFD/FGF-2 in vivo increased the formation of dentin-like material. Conclusion: FGF-2 expansion of stem cells from inflamed pulp tissues of human deciduous teeth can be a good source of stem cells for future clinical applications and a novel way of using discarded inflamed tissues.

Original languageEnglish
Pages (from-to)191-204
Number of pages14
JournalOral Diseases
Volume20
Issue number2
DOIs
Publication statusPublished - 2014 Mar 1

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Dental Pulp
Deciduous Tooth
Intercellular Signaling Peptides and Proteins
Stem Cells
Mesenchymal Stromal Cells
Transplantation
Gene Expression Profiling
Dentin

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Kim, J. C. ; Park, J. C. ; Kim, S. H. ; Im, G. I. ; Kim, B. S. ; Lee, J. B. ; Choi, E. Y. ; Song, JeSeon ; Cho, K. S. ; Kim, ChangSung. / Treatment of FGF-2 on stem cells from inflamed dental pulp tissue from human deciduous teeth. In: Oral Diseases. 2014 ; Vol. 20, No. 2. pp. 191-204.
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abstract = "Objective: The purposes of this study were to isolate and characterize stem cells from inflamed pulp tissue of human functional deciduous teeth (iSHFD) and to evaluate the influence of fibroblastic growth factor-2 (FGF-2) on the regenerative potential. Materials and methods: We successfully isolated mesenchymal stem cells (MSCs) from the inflamed dental pulp tissue of human deciduous teeth and demonstrated that their regenerative potential could be enhanced by the application of FGF-2 (20 ng ml -1 ) during ex vivo expansion. Isolated stem cells expanded in FGF-2 were characterized using a colony-forming assay, proliferation, migration, in vitro differentiation, in vivo ectopic transplantation assay, and gene expression profiling. Results: MSCs isolated from the inflamed pulp tissue of functional deciduous teeth potentially possess the qualities of those from human exfoliated deciduous teeth. FGF-2 applied to iSHFD during expansion enhanced the colony-forming efficiency of these cells, increased their proliferation and migration potential, and reduced their differentiation potential in vitro. However, the ectopic transplantation of iSHFD/FGF-2 in vivo increased the formation of dentin-like material. Conclusion: FGF-2 expansion of stem cells from inflamed pulp tissues of human deciduous teeth can be a good source of stem cells for future clinical applications and a novel way of using discarded inflamed tissues.",
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Kim, JC, Park, JC, Kim, SH, Im, GI, Kim, BS, Lee, JB, Choi, EY, Song, J, Cho, KS & Kim, C 2014, 'Treatment of FGF-2 on stem cells from inflamed dental pulp tissue from human deciduous teeth', Oral Diseases, vol. 20, no. 2, pp. 191-204. https://doi.org/10.1111/odi.12089

Treatment of FGF-2 on stem cells from inflamed dental pulp tissue from human deciduous teeth. / Kim, J. C.; Park, J. C.; Kim, S. H.; Im, G. I.; Kim, B. S.; Lee, J. B.; Choi, E. Y.; Song, JeSeon; Cho, K. S.; Kim, ChangSung.

In: Oral Diseases, Vol. 20, No. 2, 01.03.2014, p. 191-204.

Research output: Contribution to journalArticle

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AU - Kim, J. C.

AU - Park, J. C.

AU - Kim, S. H.

AU - Im, G. I.

AU - Kim, B. S.

AU - Lee, J. B.

AU - Choi, E. Y.

AU - Song, JeSeon

AU - Cho, K. S.

AU - Kim, ChangSung

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N2 - Objective: The purposes of this study were to isolate and characterize stem cells from inflamed pulp tissue of human functional deciduous teeth (iSHFD) and to evaluate the influence of fibroblastic growth factor-2 (FGF-2) on the regenerative potential. Materials and methods: We successfully isolated mesenchymal stem cells (MSCs) from the inflamed dental pulp tissue of human deciduous teeth and demonstrated that their regenerative potential could be enhanced by the application of FGF-2 (20 ng ml -1 ) during ex vivo expansion. Isolated stem cells expanded in FGF-2 were characterized using a colony-forming assay, proliferation, migration, in vitro differentiation, in vivo ectopic transplantation assay, and gene expression profiling. Results: MSCs isolated from the inflamed pulp tissue of functional deciduous teeth potentially possess the qualities of those from human exfoliated deciduous teeth. FGF-2 applied to iSHFD during expansion enhanced the colony-forming efficiency of these cells, increased their proliferation and migration potential, and reduced their differentiation potential in vitro. However, the ectopic transplantation of iSHFD/FGF-2 in vivo increased the formation of dentin-like material. Conclusion: FGF-2 expansion of stem cells from inflamed pulp tissues of human deciduous teeth can be a good source of stem cells for future clinical applications and a novel way of using discarded inflamed tissues.

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