Trifunctional Fluorogenic Probes for Fluorescence Imaging and Isolation of Glycosidases in Cells

Ji Young Hyun, Seong Hyun Park, Cheol Wan Park, Han Byeol Kim, Jin Won Cho, Injae Shin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

For both fluorescence imaging and isolation of glycosidases in cells, we prepared novel activity-based, trifunctional fluorogenic probes that consist of (1) a sugar moiety as a glycosidase substrate, (2) a fluoromethylated coumarin for fluorescent labeling, and (3) an alkyne tag for click reaction to enable isolation of the labeled enzyme. One probe, β-GlcNAc-CM-F, was employed to fluorescently detect endogenous O-GlcNAcase in cells and to isolate the labeled enzyme by affinity chromatography.

Original languageEnglish
Pages (from-to)4439-4442
Number of pages4
JournalOrganic Letters
Volume21
Issue number12
DOIs
Publication statusPublished - 2019 Jun 21

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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