Abstract
For both fluorescence imaging and isolation of glycosidases in cells, we prepared novel activity-based, trifunctional fluorogenic probes that consist of (1) a sugar moiety as a glycosidase substrate, (2) a fluoromethylated coumarin for fluorescent labeling, and (3) an alkyne tag for click reaction to enable isolation of the labeled enzyme. One probe, β-GlcNAc-CM-F, was employed to fluorescently detect endogenous O-GlcNAcase in cells and to isolate the labeled enzyme by affinity chromatography.
| Original language | English |
|---|---|
| Pages (from-to) | 4439-4442 |
| Number of pages | 4 |
| Journal | Organic Letters |
| Volume | 21 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2019 Jun 21 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Physical and Theoretical Chemistry
- Organic Chemistry
Cite this
}
Trifunctional Fluorogenic Probes for Fluorescence Imaging and Isolation of Glycosidases in Cells. / Hyun, Ji Young; Park, Seong Hyun; Park, Cheol Wan; Kim, Han Byeol; Cho, Jin Won; Shin, Injae.
In: Organic Letters, Vol. 21, No. 12, 21.06.2019, p. 4439-4442.Research output: Contribution to journal › Article
TY - JOUR
T1 - Trifunctional Fluorogenic Probes for Fluorescence Imaging and Isolation of Glycosidases in Cells
AU - Hyun, Ji Young
AU - Park, Seong Hyun
AU - Park, Cheol Wan
AU - Kim, Han Byeol
AU - Cho, Jin Won
AU - Shin, Injae
PY - 2019/6/21
Y1 - 2019/6/21
N2 - For both fluorescence imaging and isolation of glycosidases in cells, we prepared novel activity-based, trifunctional fluorogenic probes that consist of (1) a sugar moiety as a glycosidase substrate, (2) a fluoromethylated coumarin for fluorescent labeling, and (3) an alkyne tag for click reaction to enable isolation of the labeled enzyme. One probe, β-GlcNAc-CM-F, was employed to fluorescently detect endogenous O-GlcNAcase in cells and to isolate the labeled enzyme by affinity chromatography.
AB - For both fluorescence imaging and isolation of glycosidases in cells, we prepared novel activity-based, trifunctional fluorogenic probes that consist of (1) a sugar moiety as a glycosidase substrate, (2) a fluoromethylated coumarin for fluorescent labeling, and (3) an alkyne tag for click reaction to enable isolation of the labeled enzyme. One probe, β-GlcNAc-CM-F, was employed to fluorescently detect endogenous O-GlcNAcase in cells and to isolate the labeled enzyme by affinity chromatography.
UR - http://www.scopus.com/inward/record.url?scp=85065816609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065816609&partnerID=8YFLogxK
U2 - 10.1021/acs.orglett.9b01147
DO - 10.1021/acs.orglett.9b01147
M3 - Article
C2 - 31045373
AN - SCOPUS:85065816609
VL - 21
SP - 4439
EP - 4442
JO - Organic Letters
JF - Organic Letters
SN - 1523-7060
IS - 12
ER -