Trifunctional Fluorogenic Probes for Fluorescence Imaging and Isolation of Glycosidases in Cells

Ji Young Hyun, Seong Hyun Park, Cheol Wan Park, Han Byeol Kim, Jin Won Cho, Injae Shin

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

For both fluorescence imaging and isolation of glycosidases in cells, we prepared novel activity-based, trifunctional fluorogenic probes that consist of (1) a sugar moiety as a glycosidase substrate, (2) a fluoromethylated coumarin for fluorescent labeling, and (3) an alkyne tag for click reaction to enable isolation of the labeled enzyme. One probe, β-GlcNAc-CM-F, was employed to fluorescently detect endogenous O-GlcNAcase in cells and to isolate the labeled enzyme by affinity chromatography.

Original languageEnglish
Pages (from-to)4439-4442
Number of pages4
JournalOrganic Letters
Volume21
Issue number12
DOIs
Publication statusPublished - 2019 Jun 21

Bibliographical note

Funding Information:
This study was supported financially by the National Creative Research Initiative program (Grant No. 2010-0018272 to I.S.) and the National Research Foundation (Grant No. NRF-2016R1A5A1010764 to J.W.C).

Publisher Copyright:
© 2019 American Chemical Society.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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