For both fluorescence imaging and isolation of glycosidases in cells, we prepared novel activity-based, trifunctional fluorogenic probes that consist of (1) a sugar moiety as a glycosidase substrate, (2) a fluoromethylated coumarin for fluorescent labeling, and (3) an alkyne tag for click reaction to enable isolation of the labeled enzyme. One probe, β-GlcNAc-CM-F, was employed to fluorescently detect endogenous O-GlcNAcase in cells and to isolate the labeled enzyme by affinity chromatography.
Bibliographical noteFunding Information:
This study was supported financially by the National Creative Research Initiative program (Grant No. 2010-0018272 to I.S.) and the National Research Foundation (Grant No. NRF-2016R1A5A1010764 to J.W.C).
All Science Journal Classification (ASJC) codes
- Physical and Theoretical Chemistry
- Organic Chemistry