Abstract
For both fluorescence imaging and isolation of glycosidases in cells, we prepared novel activity-based, trifunctional fluorogenic probes that consist of (1) a sugar moiety as a glycosidase substrate, (2) a fluoromethylated coumarin for fluorescent labeling, and (3) an alkyne tag for click reaction to enable isolation of the labeled enzyme. One probe, β-GlcNAc-CM-F, was employed to fluorescently detect endogenous O-GlcNAcase in cells and to isolate the labeled enzyme by affinity chromatography.
| Original language | English |
|---|---|
| Pages (from-to) | 4439-4442 |
| Number of pages | 4 |
| Journal | Organic Letters |
| Volume | 21 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2019 Jun 21 |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Physical and Theoretical Chemistry
- Organic Chemistry
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Cite this
Hyun, J. Y., Park, S. H., Park, C. W., Kim, H. B., Cho, J. W., & Shin, I. (2019). Trifunctional Fluorogenic Probes for Fluorescence Imaging and Isolation of Glycosidases in Cells. Organic Letters, 21(12), 4439-4442. https://doi.org/10.1021/acs.orglett.9b01147