Triglyceride-induced macrophage cell death is triggered by caspase-1

Sin Jee Son, Kijong Rhee, Jaewon Lim, Tae Ue Kim, Tack Joong Kim, Yoon Suk Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Triglyceride (TG) induces macrophage cell death which contributes to the development of atherosclerosis. We confirmed that exogenous TG accumulates in human THP-1 macrophages and causes cell death. TG treated THP-1 macrophages exhibited no change in tumor necrosis factor (TNF)-α, interleukin (IL)-18, macrophage inflammatory protein (MIP)-1α, and IL-1R1 receptor mRNA expression. However, there was a marked decrease in IL-1β mRNA expression but an increase in IL-1β protein secretion. Decreased expression of IL-1β mRNA and increased secretion of IL-1β protein was not the direct cause of cell death. Until now, TG was assumed to induce necrotic cell death in macrophages. Since caspase-1 is known to be involved in activation and secretion of IL-1β protein and pyroptotic cell death, next we determined whether caspase-1 is associated with TG-induced macrophage cell death. We found an increase in caspase-1 activity in TG-treated THP-1 macrophages and inhibition of caspase-1 activity using a specific inhibitor partially rescued cell death. These results suggest activation of the pyroptotic pathway by TG. This is the first report implicating the activation of caspase-1 and the triggering of the pyroptosis pathway in TG-induced macrophage cell death.

Original languageEnglish
Pages (from-to)108-113
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume36
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

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Caspase 1
Triglycerides
Cell Death
Macrophages
Interleukin-1
Messenger RNA
Cause of Death
Interleukin Receptors
Macrophage Inflammatory Proteins
Proteins
Interleukin-18
Atherosclerosis
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

Son, Sin Jee ; Rhee, Kijong ; Lim, Jaewon ; Kim, Tae Ue ; Kim, Tack Joong ; Kim, Yoon Suk. / Triglyceride-induced macrophage cell death is triggered by caspase-1. In: Biological and Pharmaceutical Bulletin. 2013 ; Vol. 36, No. 1. pp. 108-113.
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Triglyceride-induced macrophage cell death is triggered by caspase-1. / Son, Sin Jee; Rhee, Kijong; Lim, Jaewon; Kim, Tae Ue; Kim, Tack Joong; Kim, Yoon Suk.

In: Biological and Pharmaceutical Bulletin, Vol. 36, No. 1, 01.01.2013, p. 108-113.

Research output: Contribution to journalArticle

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