TRIM31 promotes Atg5/Atg7-independent autophagy in intestinal cells

Eun A. Ra, Taeyun A. Lee, Seung Won Kim, Areum Park, Hyun Jin Choi, Insook Jang, Sujin Kang, Jae Hee Cheon, Jin Won Cho, Ji Eun Lee, Sungwook Lee, Boyoun Park

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Autophagy is responsible for the bulk degradation of cytosolic constituents and plays an essential role in the intestinal epithelium by controlling beneficial host-bacterial relationships. Atg5 and Atg7 are thought to be critical for autophagy. However, Atg5- or Atg7-deficient cells still form autophagosomes and autolysosomes, and are capable of removing proteins or bacteria. Here, we report that human TRIM31 (tripartite motif), an intestine-specific protein localized in mitochondria, is essential for promoting lipopolysaccharide-induced Atg5/Atg7-independent autophagy. TRIM31 directly interacts with phosphatidylethanolamine in a palmitoylation-dependent manner, leading to induction of autolysosome formation. Depletion of endogenous TRIM31 significantly increases the number of intestinal epithelial cells containing invasive bacteria. Crohn's disease patients display TRIM31 downregulation. Human cytomegalovirus-infected intestinal cells show a decrease in TRIM31 expression as well as a significant increase in bacterial load, reversible by the introduction of wild-type TRIM31. We provide insight into an alternative autophagy pathway that protects against intestinal pathogenic bacterial infection.

Original languageEnglish
Article number11726
JournalNature communications
Volume7
DOIs
Publication statusPublished - 2016 May 24

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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