Tripartite motif-containing protein 30 modulates TCR-activated proliferation and effector functions in CD4+ T cells

Un Yung Choi, Ji Yeon Hur, Myeong Sup Lee, Quanri Zhang, Won Young Choi, Lark Kyun Kim, Wook Bin Lee, Goo Taeg Oh, Young Joon Kim

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10 Citations (Scopus)

Abstract

To avoid excessive activation, immune signals are tightly controlled by diverse inhibitory proteins. TRIM30, a tripartite motif (TRIM)-containing protein is one of such inhibitors known to function in macrophages. To define the roles of TRIM30, we generated Trim30 knockout (Trim30-/-) mice. Trim30 deletion caused no major developmental defects in any organs, nor showed any discernable defect in the activation of macrophages. But, Trim30 -/- mice showed increased CD4/CD8 ratio when aged and Trim30 -/- CD4+ T cells exhibited an abnormal response upon TCR activation, in particular in the absence of a costimulatory signal. Adoptive transfer of wild-type and Trim302/2 CD4+ T cells together into lymphopenic hosts confirmed higher proliferation of the Trim30-/- CD4+ T cells in vivo. Despite the enhanced proliferation, Trim30-/- T cells showed decreased levels of NF-κB activation and IL-2 production compared to wild-type cells. These results indicate a distinct requirement for TRIM30 in modulation of NF-κB activation and cell proliferation induced by TCR stimulation.

Original languageEnglish
Article numbere95805
JournalPloS one
Volume9
Issue number4
DOIs
Publication statusPublished - 2014 Apr 22

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Choi, U. Y., Hur, J. Y., Lee, M. S., Zhang, Q., Choi, W. Y., Kim, L. K., Lee, W. B., Oh, G. T., & Kim, Y. J. (2014). Tripartite motif-containing protein 30 modulates TCR-activated proliferation and effector functions in CD4+ T cells. PloS one, 9(4), [e95805]. https://doi.org/10.1371/journal.pone.0095805