Tris(2,2′-bipyridyl)ruthenium(II) electrogenerated chemiluminescence ethanol biosensor based on ionic liquid doped titania-Nafion composite film

Yuna Yang, Sungju Nam, Wonyong Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A highly sensitive tris(2,2′-bipyridyl)ruthenium(II) [Ru(bpy)3 2+] electrogenerated chemiluminescence (ECL) biosensor for the detection of ethanol based on ADH and NAD+ cofactor with ionic liquid (IL) doped sol-gel titania-Nafion composite film has been developed. Regardless of the type of the ILs, the incorporation of IL in the composite resulted in the enhancement in both the electron transfer and diffusion rates in the composite modified electrode, thus leading to dramatic increase in ECL intensity. Among several ILs tested, 1-butyl-3-methylimidazolium with chloride counter anion was most effective for the construction of the Ru(bpy)3 2+ ECL biosensor. Two-layer system with the inner layer of Ru(bpy)3 2+ immobilized in IL-titania-Nafion composite film and the outer layer of ADH immobilized in titania-Nafion composite film has been used to maintain the activity of the enzyme and the overall stability of the ECL biosensor. The present biosensor exhibited linear response towards ethanol with a linear dynamic range of 1.2 × 10−5 M–2.5 × 10−1 M and a detection limit of 5.0 × 10−6 M (S/N = 3). The outstanding dynamic range showed the applicability of the present biosensor for ethanol detection not only in human blood but also in a variety of different fields such as alcoholic beverages or other commercially available products.

Original languageEnglish
Pages (from-to)62-69
Number of pages8
JournalMicrochemical Journal
Volume142
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

Ionic Liquids
2,2'-Dipyridyl
Chemiluminescence
Ruthenium
Composite films
Biosensors
Ethanol
Composite materials
Interleukin-1
NAD
Sol-gels
Anions
Chlorides
Blood
perfluorosulfonic acid
titanium dioxide
Electrodes
Electrons
Enzymes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy

Cite this

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title = "Tris(2,2′-bipyridyl)ruthenium(II) electrogenerated chemiluminescence ethanol biosensor based on ionic liquid doped titania-Nafion composite film",
abstract = "A highly sensitive tris(2,2′-bipyridyl)ruthenium(II) [Ru(bpy)3 2+] electrogenerated chemiluminescence (ECL) biosensor for the detection of ethanol based on ADH and NAD+ cofactor with ionic liquid (IL) doped sol-gel titania-Nafion composite film has been developed. Regardless of the type of the ILs, the incorporation of IL in the composite resulted in the enhancement in both the electron transfer and diffusion rates in the composite modified electrode, thus leading to dramatic increase in ECL intensity. Among several ILs tested, 1-butyl-3-methylimidazolium with chloride counter anion was most effective for the construction of the Ru(bpy)3 2+ ECL biosensor. Two-layer system with the inner layer of Ru(bpy)3 2+ immobilized in IL-titania-Nafion composite film and the outer layer of ADH immobilized in titania-Nafion composite film has been used to maintain the activity of the enzyme and the overall stability of the ECL biosensor. The present biosensor exhibited linear response towards ethanol with a linear dynamic range of 1.2 × 10−5 M–2.5 × 10−1 M and a detection limit of 5.0 × 10−6 M (S/N = 3). The outstanding dynamic range showed the applicability of the present biosensor for ethanol detection not only in human blood but also in a variety of different fields such as alcoholic beverages or other commercially available products.",
author = "Yuna Yang and Sungju Nam and Wonyong Lee",
year = "2018",
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T1 - Tris(2,2′-bipyridyl)ruthenium(II) electrogenerated chemiluminescence ethanol biosensor based on ionic liquid doped titania-Nafion composite film

AU - Yang, Yuna

AU - Nam, Sungju

AU - Lee, Wonyong

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