Expression of a Rhodococcus-derived oxygenase gene in Escherichia coli yielded indigo metabolites with cytotoxic activity against cancer cells. Bioactivity-guided fractionation of these indigo metabolites led to the isolation of trisindoline as the agent responsible for the observed in vitro cytotoxic activity against cancer cells. While the cytotoxicity of etoposide, a common anticancer drug, was dramatically decreased in multidrug-resistant (MDR) cancer cells compared with treatment of parental cells, trisindoline was found to have similar cytotoxicity effects on both parental and MDR cell lines. In addition, the cytotoxic effects of trisindoline were resistant to P-glycoprotein overexpression, one of the most common mechanisms of drug resistance in cancer cells, supporting its use to kill MDR cancer cells.
|Number of pages||4|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2008 Nov 7|
Bibliographical noteFunding Information:
This work was supported by a grant from the Ministry of Education, Science, and Technology, Republic of Korea, to EK through the 21C Frontier Microbial Genomics and Applications Center Program and also by a grant from KOSEF through the Advanced Environmental Biotechnology Research Center at POSTECH. K.C. is a recipient of the Brain Korea 21 scholarship.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology