Abstract
Expression of a Rhodococcus-derived oxygenase gene in Escherichia coli yielded indigo metabolites with cytotoxic activity against cancer cells. Bioactivity-guided fractionation of these indigo metabolites led to the isolation of trisindoline as the agent responsible for the observed in vitro cytotoxic activity against cancer cells. While the cytotoxicity of etoposide, a common anticancer drug, was dramatically decreased in multidrug-resistant (MDR) cancer cells compared with treatment of parental cells, trisindoline was found to have similar cytotoxicity effects on both parental and MDR cell lines. In addition, the cytotoxic effects of trisindoline were resistant to P-glycoprotein overexpression, one of the most common mechanisms of drug resistance in cancer cells, supporting its use to kill MDR cancer cells.
| Original language | English |
|---|---|
| Pages (from-to) | 96-99 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 376 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 Nov 7 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
}
Trisindoline synthesis and anticancer activity. / Yoo, Miyoun; Choi, Sang Un; Choi, Ki Young; Yon, Gyu Hwan; Chae, Jong Chan; Kim, Dockyu; Zylstra, Gerben J.; Kim, Eungbin.
In: Biochemical and Biophysical Research Communications, Vol. 376, No. 1, 07.11.2008, p. 96-99.Research output: Contribution to journal › Article
TY - JOUR
T1 - Trisindoline synthesis and anticancer activity
AU - Yoo, Miyoun
AU - Choi, Sang Un
AU - Choi, Ki Young
AU - Yon, Gyu Hwan
AU - Chae, Jong Chan
AU - Kim, Dockyu
AU - Zylstra, Gerben J.
AU - Kim, Eungbin
PY - 2008/11/7
Y1 - 2008/11/7
N2 - Expression of a Rhodococcus-derived oxygenase gene in Escherichia coli yielded indigo metabolites with cytotoxic activity against cancer cells. Bioactivity-guided fractionation of these indigo metabolites led to the isolation of trisindoline as the agent responsible for the observed in vitro cytotoxic activity against cancer cells. While the cytotoxicity of etoposide, a common anticancer drug, was dramatically decreased in multidrug-resistant (MDR) cancer cells compared with treatment of parental cells, trisindoline was found to have similar cytotoxicity effects on both parental and MDR cell lines. In addition, the cytotoxic effects of trisindoline were resistant to P-glycoprotein overexpression, one of the most common mechanisms of drug resistance in cancer cells, supporting its use to kill MDR cancer cells.
AB - Expression of a Rhodococcus-derived oxygenase gene in Escherichia coli yielded indigo metabolites with cytotoxic activity against cancer cells. Bioactivity-guided fractionation of these indigo metabolites led to the isolation of trisindoline as the agent responsible for the observed in vitro cytotoxic activity against cancer cells. While the cytotoxicity of etoposide, a common anticancer drug, was dramatically decreased in multidrug-resistant (MDR) cancer cells compared with treatment of parental cells, trisindoline was found to have similar cytotoxicity effects on both parental and MDR cell lines. In addition, the cytotoxic effects of trisindoline were resistant to P-glycoprotein overexpression, one of the most common mechanisms of drug resistance in cancer cells, supporting its use to kill MDR cancer cells.
UR - http://www.scopus.com/inward/record.url?scp=52049112527&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52049112527&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.08.092
DO - 10.1016/j.bbrc.2008.08.092
M3 - Article
C2 - 18760995
AN - SCOPUS:52049112527
VL - 376
SP - 96
EP - 99
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -