Trisindoline synthesis and anticancer activity

Miyoun Yoo, Sang Un Choi, Ki Young Choi, Gyu Hwan Yon, Jong Chan Chae, Dockyu Kim, Gerben J. Zylstra, Eungbin Kim

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19 Citations (Scopus)

Abstract

Expression of a Rhodococcus-derived oxygenase gene in Escherichia coli yielded indigo metabolites with cytotoxic activity against cancer cells. Bioactivity-guided fractionation of these indigo metabolites led to the isolation of trisindoline as the agent responsible for the observed in vitro cytotoxic activity against cancer cells. While the cytotoxicity of etoposide, a common anticancer drug, was dramatically decreased in multidrug-resistant (MDR) cancer cells compared with treatment of parental cells, trisindoline was found to have similar cytotoxicity effects on both parental and MDR cell lines. In addition, the cytotoxic effects of trisindoline were resistant to P-glycoprotein overexpression, one of the most common mechanisms of drug resistance in cancer cells, supporting its use to kill MDR cancer cells.

Original languageEnglish
Pages (from-to)96-99
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume376
Issue number1
DOIs
Publication statusPublished - 2008 Nov 7

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yoo, M., Choi, S. U., Choi, K. Y., Yon, G. H., Chae, J. C., Kim, D., Zylstra, G. J., & Kim, E. (2008). Trisindoline synthesis and anticancer activity. Biochemical and Biophysical Research Communications, 376(1), 96-99. https://doi.org/10.1016/j.bbrc.2008.08.092