TRPC3 channels confer cellular memory of recent neuromuscular activity

Paul Rosenberg, April Hawkins, Jonathan Stiber, John M. Shelton, Kelley Hutcheson, Rhonda Bassel-Duby, Dong Min Shin, Zhen Yan, R. Sanders Williams

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Skeletal muscle adapts to different patterns of motor nerve activity by alterations in gene expression that match specialized properties of contraction, metabolism, and muscle mass to changing work demands (muscle plasticity). Calcineurin, a calcium/calmodulin-dependent, serine-threonine protein phosphatase, has been shown to control programs of gene expression in skeletal muscles, as in other cell types, through the transcription factor nuclear factor of activated T cells (NFAT). This study provides evidence that the function of NFAT as a transcriptional activator is regulated by neuromuscular stimulation in muscles of intact animals and that calcium influx from the transient receptor potential (TRPC3) channel is an important determinant of NFAT activity. Expression of TRPC3 channels in skeletal myocytes is up-regulated by neuromuscular activity in a calcineurin-dependent manner. These data suggest a mechanism for cellular memory in skeletal muscles whereby repeated bouts of contractile activity drive progressively greater remodeling events.

Original languageEnglish
Pages (from-to)9387-9392
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number25
DOIs
Publication statusPublished - 2004 Jun 22

Fingerprint

NFATC Transcription Factors
Skeletal Muscle
Calcineurin
Calcium
Transient Receptor Potential Channels
Gene Expression
Muscles
Phosphoprotein Phosphatases
Skeletal Muscle Fibers
Calmodulin
Muscle Contraction
Motor Activity
Transcription Factors
TRPC3 cation channel

All Science Journal Classification (ASJC) codes

  • General

Cite this

Rosenberg, P., Hawkins, A., Stiber, J., Shelton, J. M., Hutcheson, K., Bassel-Duby, R., ... Williams, R. S. (2004). TRPC3 channels confer cellular memory of recent neuromuscular activity. Proceedings of the National Academy of Sciences of the United States of America, 101(25), 9387-9392. https://doi.org/10.1073/pnas.0308179101
Rosenberg, Paul ; Hawkins, April ; Stiber, Jonathan ; Shelton, John M. ; Hutcheson, Kelley ; Bassel-Duby, Rhonda ; Shin, Dong Min ; Yan, Zhen ; Williams, R. Sanders. / TRPC3 channels confer cellular memory of recent neuromuscular activity. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 25. pp. 9387-9392.
@article{05642c3e790a4c989c030654b1bf8e22,
title = "TRPC3 channels confer cellular memory of recent neuromuscular activity",
abstract = "Skeletal muscle adapts to different patterns of motor nerve activity by alterations in gene expression that match specialized properties of contraction, metabolism, and muscle mass to changing work demands (muscle plasticity). Calcineurin, a calcium/calmodulin-dependent, serine-threonine protein phosphatase, has been shown to control programs of gene expression in skeletal muscles, as in other cell types, through the transcription factor nuclear factor of activated T cells (NFAT). This study provides evidence that the function of NFAT as a transcriptional activator is regulated by neuromuscular stimulation in muscles of intact animals and that calcium influx from the transient receptor potential (TRPC3) channel is an important determinant of NFAT activity. Expression of TRPC3 channels in skeletal myocytes is up-regulated by neuromuscular activity in a calcineurin-dependent manner. These data suggest a mechanism for cellular memory in skeletal muscles whereby repeated bouts of contractile activity drive progressively greater remodeling events.",
author = "Paul Rosenberg and April Hawkins and Jonathan Stiber and Shelton, {John M.} and Kelley Hutcheson and Rhonda Bassel-Duby and Shin, {Dong Min} and Zhen Yan and Williams, {R. Sanders}",
year = "2004",
month = "6",
day = "22",
doi = "10.1073/pnas.0308179101",
language = "English",
volume = "101",
pages = "9387--9392",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "25",

}

Rosenberg, P, Hawkins, A, Stiber, J, Shelton, JM, Hutcheson, K, Bassel-Duby, R, Shin, DM, Yan, Z & Williams, RS 2004, 'TRPC3 channels confer cellular memory of recent neuromuscular activity', Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 25, pp. 9387-9392. https://doi.org/10.1073/pnas.0308179101

TRPC3 channels confer cellular memory of recent neuromuscular activity. / Rosenberg, Paul; Hawkins, April; Stiber, Jonathan; Shelton, John M.; Hutcheson, Kelley; Bassel-Duby, Rhonda; Shin, Dong Min; Yan, Zhen; Williams, R. Sanders.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 25, 22.06.2004, p. 9387-9392.

Research output: Contribution to journalArticle

TY - JOUR

T1 - TRPC3 channels confer cellular memory of recent neuromuscular activity

AU - Rosenberg, Paul

AU - Hawkins, April

AU - Stiber, Jonathan

AU - Shelton, John M.

AU - Hutcheson, Kelley

AU - Bassel-Duby, Rhonda

AU - Shin, Dong Min

AU - Yan, Zhen

AU - Williams, R. Sanders

PY - 2004/6/22

Y1 - 2004/6/22

N2 - Skeletal muscle adapts to different patterns of motor nerve activity by alterations in gene expression that match specialized properties of contraction, metabolism, and muscle mass to changing work demands (muscle plasticity). Calcineurin, a calcium/calmodulin-dependent, serine-threonine protein phosphatase, has been shown to control programs of gene expression in skeletal muscles, as in other cell types, through the transcription factor nuclear factor of activated T cells (NFAT). This study provides evidence that the function of NFAT as a transcriptional activator is regulated by neuromuscular stimulation in muscles of intact animals and that calcium influx from the transient receptor potential (TRPC3) channel is an important determinant of NFAT activity. Expression of TRPC3 channels in skeletal myocytes is up-regulated by neuromuscular activity in a calcineurin-dependent manner. These data suggest a mechanism for cellular memory in skeletal muscles whereby repeated bouts of contractile activity drive progressively greater remodeling events.

AB - Skeletal muscle adapts to different patterns of motor nerve activity by alterations in gene expression that match specialized properties of contraction, metabolism, and muscle mass to changing work demands (muscle plasticity). Calcineurin, a calcium/calmodulin-dependent, serine-threonine protein phosphatase, has been shown to control programs of gene expression in skeletal muscles, as in other cell types, through the transcription factor nuclear factor of activated T cells (NFAT). This study provides evidence that the function of NFAT as a transcriptional activator is regulated by neuromuscular stimulation in muscles of intact animals and that calcium influx from the transient receptor potential (TRPC3) channel is an important determinant of NFAT activity. Expression of TRPC3 channels in skeletal myocytes is up-regulated by neuromuscular activity in a calcineurin-dependent manner. These data suggest a mechanism for cellular memory in skeletal muscles whereby repeated bouts of contractile activity drive progressively greater remodeling events.

UR - http://www.scopus.com/inward/record.url?scp=3042681486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042681486&partnerID=8YFLogxK

U2 - 10.1073/pnas.0308179101

DO - 10.1073/pnas.0308179101

M3 - Article

C2 - 15199180

AN - SCOPUS:3042681486

VL - 101

SP - 9387

EP - 9392

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 25

ER -