TSLP Is a Potential Initiator of Collagen Synthesis and an Activator of CXCR4/SDF-1 Axis in Keloid Pathogenesis

Jung U. Shin, Seo Hyeong Kim, Hyeran Kim, Ji Yeon Noh, Shan Jin, Chang Ook Park, Won Jai Lee, Dong Won Lee, Ju Hee Lee, Kwang Hoon Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recently, thymic stromal lymphopoietin (TSLP), which is well studied in allergic diseases, has been reported in fibrotic diseases, including idiopathic pulmonary fibrosis and atopic dermatitis fibrosis. However, the role of TSLP in keloid is obscure. In this study, we assessed the expression of TSLP in keloid tissue and investigated the possible role of TSLP in keloid pathogenesis. We observed that TSLP expression was increased in keloid tissue compared to normal tissue. Furthermore, TSLP treatment induced increased collagen I and collagen III expression in fibroblasts via transforming growth factor-β; however, there was higher expression in keloid fibroblasts compared to normal fibroblasts. Stromal cell-derived factor-1α, which was recently reported to enhance wound healing through recruiting bone marrow-derived mesenchymal stem cells to the wound area, increased after TSLP treatment in fibroblasts and was primarily expressed in α-smooth muscle action-positive myofibroblasts in keloid tissue. Furthermore, fibrocytes expressing CXCR4, a stromal cell-derived factor-1α receptor, were significantly increased in keloid tissue compared to normal tissue. Finally, intradermal TSLP injection on BALB/c mice increased stromal cell-derived factor-1α expression and CXCR4+ fibrocytes infiltration. Our data suggest that TSLP is a potent inducer of collagen and transforming growth factor-β production in keloid fibroblasts. In addition, it might activate the CXCR4/stromal cell-derived factor-1 axis to increase fibrocyte infiltration into the keloid tissue.

Original languageEnglish
Pages (from-to)507-515
Number of pages9
JournalJournal of Investigative Dermatology
Volume136
Issue number2
DOIs
Publication statusPublished - 2016 Jan 1

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Keloid
Collagen
Fibroblasts
Chemokine CXCL12
Tissue
Transforming Growth Factors
Infiltration
thymic stromal lymphopoietin
Idiopathic Pulmonary Fibrosis
Myofibroblasts
Atopic Dermatitis
Stem cells
Mesenchymal Stromal Cells
Wound Healing
Smooth Muscle
Muscle
Bone
Fibrosis
Bone Marrow
Injections

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Shin, Jung U. ; Kim, Seo Hyeong ; Kim, Hyeran ; Noh, Ji Yeon ; Jin, Shan ; Park, Chang Ook ; Lee, Won Jai ; Lee, Dong Won ; Lee, Ju Hee ; Lee, Kwang Hoon. / TSLP Is a Potential Initiator of Collagen Synthesis and an Activator of CXCR4/SDF-1 Axis in Keloid Pathogenesis. In: Journal of Investigative Dermatology. 2016 ; Vol. 136, No. 2. pp. 507-515.
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abstract = "Recently, thymic stromal lymphopoietin (TSLP), which is well studied in allergic diseases, has been reported in fibrotic diseases, including idiopathic pulmonary fibrosis and atopic dermatitis fibrosis. However, the role of TSLP in keloid is obscure. In this study, we assessed the expression of TSLP in keloid tissue and investigated the possible role of TSLP in keloid pathogenesis. We observed that TSLP expression was increased in keloid tissue compared to normal tissue. Furthermore, TSLP treatment induced increased collagen I and collagen III expression in fibroblasts via transforming growth factor-β; however, there was higher expression in keloid fibroblasts compared to normal fibroblasts. Stromal cell-derived factor-1α, which was recently reported to enhance wound healing through recruiting bone marrow-derived mesenchymal stem cells to the wound area, increased after TSLP treatment in fibroblasts and was primarily expressed in α-smooth muscle action-positive myofibroblasts in keloid tissue. Furthermore, fibrocytes expressing CXCR4, a stromal cell-derived factor-1α receptor, were significantly increased in keloid tissue compared to normal tissue. Finally, intradermal TSLP injection on BALB/c mice increased stromal cell-derived factor-1α expression and CXCR4+ fibrocytes infiltration. Our data suggest that TSLP is a potent inducer of collagen and transforming growth factor-β production in keloid fibroblasts. In addition, it might activate the CXCR4/stromal cell-derived factor-1 axis to increase fibrocyte infiltration into the keloid tissue.",
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TSLP Is a Potential Initiator of Collagen Synthesis and an Activator of CXCR4/SDF-1 Axis in Keloid Pathogenesis. / Shin, Jung U.; Kim, Seo Hyeong; Kim, Hyeran; Noh, Ji Yeon; Jin, Shan; Park, Chang Ook; Lee, Won Jai; Lee, Dong Won; Lee, Ju Hee; Lee, Kwang Hoon.

In: Journal of Investigative Dermatology, Vol. 136, No. 2, 01.01.2016, p. 507-515.

Research output: Contribution to journalArticle

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