Two cation transporters Ena1 and Nha1 cooperatively modulate ion homeostasis, antifungal drug resistance, and virulence of Cryptococcus neoformans via the HOG pathway

Kwang Woo Jung, Anna K. Strain, Kirsten Nielsen, Kwang Hwan Jung, Yong Sun Bahn

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Maintenance of cation homeostasis is essential for survival of all living organisms in their biological niches. It is also important for the survival of human pathogenic fungi in the host, where cation concentrations and pH will vary depending on different anatomical sites. However, the exact role of diverse cation transporters and ion channels in virulence of fungal pathogens remains elusive. In this study we functionally characterized ENA1 and NHA1, encoding a putative Na +/ATPase and Na +/H + antiporter, respectively, in Cryptococcus neoformans, a basidiomycete fungal pathogen which causes fatal meningoencephalitis. Expression of NHA1 and ENA1 is induced in response to salt and osmotic shock mainly in a Hog1-dependent manner. Phenotypic analysis of the ena1Δ, nha1Δ, and ena1Δnha1Δ mutants revealed that Ena1 controls cellular levels of toxic cations, such as Na + and Li + whereas both Ena1 and Nha1 are important for controlling less toxic K + ions. Under alkaline conditions, Ena1 was highly induced and required for growth in the presence of low levels of Na + or K + salt and Nha1 played a role in survival under K + stress. In contrast, Nha1, but not Ena1, was essential for survival at acidic conditions (pH 4.5) under high K + stress. In addition, Ena1 and Nha1 were required for maintenance of plasma membrane potential and stability, which appeared to modulate antifungal drug susceptibility. Perturbation of ENA1 and NHA1 enhanced capsule production and melanin synthesis. However, Nha1 was dispensable for virulence of C. neoformans although Ena1 was essential. In conclusion, Ena1 and Nha1 play redundant and discrete roles in cation homeostasis, pH regulation, membrane potential, and virulence in C. neoformans, suggesting that these transporters could be novel antifungal drug targets for treatment of cryptococcosis.

Original languageEnglish
Pages (from-to)332-345
Number of pages14
JournalFungal Genetics and Biology
Volume49
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

Fingerprint

Fungal Drug Resistance
Cryptococcus neoformans
Virulence
Cations
Homeostasis
Ions
Poisons
Membrane Potentials
Survival
Salts
Maintenance
Cryptococcosis
Basidiomycota
Sodium-Hydrogen Antiporter
Meningoencephalitis
Melanins
Osmotic Pressure
Ion Channels
Pharmaceutical Preparations
Capsules

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

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title = "Two cation transporters Ena1 and Nha1 cooperatively modulate ion homeostasis, antifungal drug resistance, and virulence of Cryptococcus neoformans via the HOG pathway",
abstract = "Maintenance of cation homeostasis is essential for survival of all living organisms in their biological niches. It is also important for the survival of human pathogenic fungi in the host, where cation concentrations and pH will vary depending on different anatomical sites. However, the exact role of diverse cation transporters and ion channels in virulence of fungal pathogens remains elusive. In this study we functionally characterized ENA1 and NHA1, encoding a putative Na +/ATPase and Na +/H + antiporter, respectively, in Cryptococcus neoformans, a basidiomycete fungal pathogen which causes fatal meningoencephalitis. Expression of NHA1 and ENA1 is induced in response to salt and osmotic shock mainly in a Hog1-dependent manner. Phenotypic analysis of the ena1Δ, nha1Δ, and ena1Δnha1Δ mutants revealed that Ena1 controls cellular levels of toxic cations, such as Na + and Li + whereas both Ena1 and Nha1 are important for controlling less toxic K + ions. Under alkaline conditions, Ena1 was highly induced and required for growth in the presence of low levels of Na + or K + salt and Nha1 played a role in survival under K + stress. In contrast, Nha1, but not Ena1, was essential for survival at acidic conditions (pH 4.5) under high K + stress. In addition, Ena1 and Nha1 were required for maintenance of plasma membrane potential and stability, which appeared to modulate antifungal drug susceptibility. Perturbation of ENA1 and NHA1 enhanced capsule production and melanin synthesis. However, Nha1 was dispensable for virulence of C. neoformans although Ena1 was essential. In conclusion, Ena1 and Nha1 play redundant and discrete roles in cation homeostasis, pH regulation, membrane potential, and virulence in C. neoformans, suggesting that these transporters could be novel antifungal drug targets for treatment of cryptococcosis.",
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Two cation transporters Ena1 and Nha1 cooperatively modulate ion homeostasis, antifungal drug resistance, and virulence of Cryptococcus neoformans via the HOG pathway. / Jung, Kwang Woo; Strain, Anna K.; Nielsen, Kirsten; Jung, Kwang Hwan; Bahn, Yong Sun.

In: Fungal Genetics and Biology, Vol. 49, No. 4, 01.04.2012, p. 332-345.

Research output: Contribution to journalArticle

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