Two-dimensional electrophoretic profiling of atopic dermatitis in primary cultured keratinocytes from patients

Yong Doo Park, Hee Sun Jang, So Yeon Kim, Sung Kyun Ko, You Jeong Lyou, Dong Youn Lee, Young Ki Paik, Jun Mo Yang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Recently, we reported altered protein expression in primary cultured fibroblasts from atopic dermatitis (AD) patients. As a sequential study, we conducted proteomic analysis of primary keratinocytes derived from AD patients to further identify AD-related proteins. Three pH ranges, 4-7, 6-9, and 7-11, were used to profile the altered protein expression in AD. We obtained 46 candidate spots from the 2-D gel image analysis: 18 proteins were up-regulated and 27 proteins were down-regulated. Among the several important candidate proteins, NCC27 showed the same profile of a defect in PTM in both AD-derived keratinocytes and fibroblasts. On the basis of current and previous reports, real-time PCR was performed on select candidate genes to compare RNA and protein expression levels in AD-derived keratinocytes and fibroblasts. Our results provide new clues to aid in understanding the mechanism of atopic alterations in keratinocytes and suggest new AD-associated proteins that are important in AD pathogenesis.

Original languageEnglish
Pages (from-to)1362-1370
Number of pages9
JournalProteomics
Volume6
Issue number4
DOIs
Publication statusPublished - 2006 Feb 1

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Atopic Dermatitis
Keratinocytes
Proteins
Fibroblasts
Pulse time modulation
Proteomics
Image analysis
Real-Time Polymerase Chain Reaction
Genes
Gels
RNA
Defects

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Park, Y. D., Jang, H. S., Kim, S. Y., Ko, S. K., Lyou, Y. J., Lee, D. Y., ... Yang, J. M. (2006). Two-dimensional electrophoretic profiling of atopic dermatitis in primary cultured keratinocytes from patients. Proteomics, 6(4), 1362-1370. https://doi.org/10.1002/pmic.200500277
Park, Yong Doo ; Jang, Hee Sun ; Kim, So Yeon ; Ko, Sung Kyun ; Lyou, You Jeong ; Lee, Dong Youn ; Paik, Young Ki ; Yang, Jun Mo. / Two-dimensional electrophoretic profiling of atopic dermatitis in primary cultured keratinocytes from patients. In: Proteomics. 2006 ; Vol. 6, No. 4. pp. 1362-1370.
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Two-dimensional electrophoretic profiling of atopic dermatitis in primary cultured keratinocytes from patients. / Park, Yong Doo; Jang, Hee Sun; Kim, So Yeon; Ko, Sung Kyun; Lyou, You Jeong; Lee, Dong Youn; Paik, Young Ki; Yang, Jun Mo.

In: Proteomics, Vol. 6, No. 4, 01.02.2006, p. 1362-1370.

Research output: Contribution to journalArticle

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