Two Photon Fluorescence Microscopy of the Unstained Human Cochlea Reveals Organ of Corti Cytoarchitecture

Janani S. Iyer; Richard Seist; In Seok Moon; Konstantina M. Stankovic

doi:10.3389/fncel.2021.690953

Two Photon Fluorescence Microscopy of the Unstained Human Cochlea Reveals Organ of Corti Cytoarchitecture

Janani S. Iyer, Richard Seist, In Seok Moon, Konstantina M. Stankovic

Department of Otorhinolaryngology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Sensorineural hearing loss (SNHL) is the most common sensory deficit worldwide, and it typically originates from the cochlea. Methods to visualize intracochlear cells in living people are currently lacking, limiting not only diagnostics but also therapies for SNHL. Two-photon fluorescence microscopy (TPFM) is a high-resolution optical imaging technique. Here we demonstrate that TPFM enables visualization of sensory cells and auditory nerve fibers in an unstained, non-decalcified adult human cochlea.

Original language	English
Article number	690953
Journal	Frontiers in Cellular Neuroscience
Volume	15
DOIs	https://doi.org/10.3389/fncel.2021.690953
Publication status	Published - 2021 Aug 5

Bibliographical note

Funding Information:
The authors are grateful for support from the Department of Defense National Defense Science and Engineering Graduate Fellowship (JI), the Lauer Tinnitus Research Center (KS), and the Bertarelli Program in Translational Neuroscience and Neuroengineering (KS).

Publisher Copyright:
© Copyright © 2021 Iyer, Seist, Moon and Stankovic.

All Science Journal Classification (ASJC) codes

Cellular and Molecular Neuroscience

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10.3389/fncel.2021.690953

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title = "Two Photon Fluorescence Microscopy of the Unstained Human Cochlea Reveals Organ of Corti Cytoarchitecture",

abstract = "Sensorineural hearing loss (SNHL) is the most common sensory deficit worldwide, and it typically originates from the cochlea. Methods to visualize intracochlear cells in living people are currently lacking, limiting not only diagnostics but also therapies for SNHL. Two-photon fluorescence microscopy (TPFM) is a high-resolution optical imaging technique. Here we demonstrate that TPFM enables visualization of sensory cells and auditory nerve fibers in an unstained, non-decalcified adult human cochlea.",

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AU - Iyer, Janani S.

AU - Seist, Richard

AU - Moon, In Seok

AU - Stankovic, Konstantina M.

N1 - Funding Information: The authors are grateful for support from the Department of Defense National Defense Science and Engineering Graduate Fellowship (JI), the Lauer Tinnitus Research Center (KS), and the Bertarelli Program in Translational Neuroscience and Neuroengineering (KS). Publisher Copyright: © Copyright © 2021 Iyer, Seist, Moon and Stankovic.

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AB - Sensorineural hearing loss (SNHL) is the most common sensory deficit worldwide, and it typically originates from the cochlea. Methods to visualize intracochlear cells in living people are currently lacking, limiting not only diagnostics but also therapies for SNHL. Two-photon fluorescence microscopy (TPFM) is a high-resolution optical imaging technique. Here we demonstrate that TPFM enables visualization of sensory cells and auditory nerve fibers in an unstained, non-decalcified adult human cochlea.

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Two Photon Fluorescence Microscopy of the Unstained Human Cochlea Reveals Organ of Corti Cytoarchitecture

Abstract

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