Type 2 immunity plays an essential role for murine model of allergic contact dermatitis with mixed type 1/type 2 immune response

Jeyun Park; Jae Won Lee; Sung Hee Kim; Jongwook Oh; Won Seok Roh; Soo Min Kim; Chang Ook Park; Min Geol Lee; Tae Gyun Kim

doi:10.1016/j.jdermsci.2021.10.001

Type 2 immunity plays an essential role for murine model of allergic contact dermatitis with mixed type 1/type 2 immune response

Jeyun Park, Jae Won Lee, Sung Hee Kim, Jongwook Oh, Won Seok Roh, Soo Min Kim, Chang Ook Park, Min Geol Lee, Tae Gyun Kim

Department of Dermatology

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Both human and mouse allergic contact dermatitis (ACD) frequently demonstrates a combined type 1 and type 2 immune response. However, the relative importance of type 2 immunity in this setting has been incompletely understood yet. Objective: To explore an effector function of type 2 immunity in ACD with mixed type 1/type 2 immune response. Methods: Gene expression characteristics of contact hypersensitivity (CHS) model was examined by quantitative polymerase chain reaction. Cytokine profile of T cells was analyzed by flow cytometry. The involvement of type 2 immunity was assessed by antibody-mediated cytokine neutralization and cell depletion. The role of specific subset of cutaneous dendritic cells was evaluated using diphtheria toxin-induced cell-depleting mouse strains. Results: Oxazolone-induced CHS revealed a combination of type 1/type 2 gene expression. The severity of oxazolone-induced CHS was ameliorated by neutralization of IL-4 but not of IFN-γ, indicating that type 2 immunity plays a dominant effector function in this mixed type 1/type 2 model. Mechanistically, type 2 effector immunity was mounted by CD301b+Langeirn− dermal dendritic cells in part through thymic stromal lymphopoietin-interleukin 7 receptor alpha signaling-dependent manner. Conclusion: Our findings suggest the clinical rationale for targeting type 2 immunity as a relevant therapeutic strategy for the mixed immune phenotype of ACD.

Original language	English
Pages (from-to)	122-131
Number of pages	10
Journal	Journal of Dermatological Science
Volume	104
Issue number	2
DOIs	https://doi.org/10.1016/j.jdermsci.2021.10.001
Publication status	Published - 2021 Nov

Bibliographical note

Funding Information:
This research was supported by a Basic Science Research Program through the National Research Foundation of Republic of Korea funded by the Ministry of Education ( 2019R1A6A1A03032869 ) and Ministry of Science and Information and Communications Technology ( 2019M3A9E8022135, 2020R1C1C1014513 ), by a Korea Health Technology R&D Project through the Korea Health Industry Development Institute funded by the Ministry of Health and Welfare ( HP20C0019, HP20C0171 ) and Korea Centers for Disease Control and Prevention ( 2020-ER6714-00 ), and by a faculty research grant of Yonsei University College of Medicine ( 6-2018-0046 ).

Publisher Copyright:
© 2021 Japanese Society for Investigative Dermatology

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Dermatology

Access to Document

10.1016/j.jdermsci.2021.10.001

Cite this

@article{0ffb67f46c0c48bab7f71e00c1b6f571,

title = "Type 2 immunity plays an essential role for murine model of allergic contact dermatitis with mixed type 1/type 2 immune response",

abstract = "Background: Both human and mouse allergic contact dermatitis (ACD) frequently demonstrates a combined type 1 and type 2 immune response. However, the relative importance of type 2 immunity in this setting has been incompletely understood yet. Objective: To explore an effector function of type 2 immunity in ACD with mixed type 1/type 2 immune response. Methods: Gene expression characteristics of contact hypersensitivity (CHS) model was examined by quantitative polymerase chain reaction. Cytokine profile of T cells was analyzed by flow cytometry. The involvement of type 2 immunity was assessed by antibody-mediated cytokine neutralization and cell depletion. The role of specific subset of cutaneous dendritic cells was evaluated using diphtheria toxin-induced cell-depleting mouse strains. Results: Oxazolone-induced CHS revealed a combination of type 1/type 2 gene expression. The severity of oxazolone-induced CHS was ameliorated by neutralization of IL-4 but not of IFN-γ, indicating that type 2 immunity plays a dominant effector function in this mixed type 1/type 2 model. Mechanistically, type 2 effector immunity was mounted by CD301b+Langeirn− dermal dendritic cells in part through thymic stromal lymphopoietin-interleukin 7 receptor alpha signaling-dependent manner. Conclusion: Our findings suggest the clinical rationale for targeting type 2 immunity as a relevant therapeutic strategy for the mixed immune phenotype of ACD.",

author = "Jeyun Park and Lee, {Jae Won} and Kim, {Sung Hee} and Jongwook Oh and Roh, {Won Seok} and Kim, {Soo Min} and Park, {Chang Ook} and Lee, {Min Geol} and Kim, {Tae Gyun}",

note = "Funding Information: This research was supported by a Basic Science Research Program through the National Research Foundation of Republic of Korea funded by the Ministry of Education ( 2019R1A6A1A03032869 ) and Ministry of Science and Information and Communications Technology ( 2019M3A9E8022135, 2020R1C1C1014513 ), by a Korea Health Technology R&D Project through the Korea Health Industry Development Institute funded by the Ministry of Health and Welfare ( HP20C0019, HP20C0171 ) and Korea Centers for Disease Control and Prevention ( 2020-ER6714-00 ), and by a faculty research grant of Yonsei University College of Medicine ( 6-2018-0046 ). Publisher Copyright: {\textcopyright} 2021 Japanese Society for Investigative Dermatology",

year = "2021",

month = nov,

doi = "10.1016/j.jdermsci.2021.10.001",

language = "English",

volume = "104",

pages = "122--131",

journal = "Journal of Dermatological Science",

issn = "0923-1811",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Type 2 immunity plays an essential role for murine model of allergic contact dermatitis with mixed type 1/type 2 immune response

AU - Park, Jeyun

AU - Lee, Jae Won

AU - Kim, Sung Hee

AU - Oh, Jongwook

AU - Roh, Won Seok

AU - Kim, Soo Min

AU - Park, Chang Ook

AU - Lee, Min Geol

AU - Kim, Tae Gyun

N1 - Funding Information: This research was supported by a Basic Science Research Program through the National Research Foundation of Republic of Korea funded by the Ministry of Education ( 2019R1A6A1A03032869 ) and Ministry of Science and Information and Communications Technology ( 2019M3A9E8022135, 2020R1C1C1014513 ), by a Korea Health Technology R&D Project through the Korea Health Industry Development Institute funded by the Ministry of Health and Welfare ( HP20C0019, HP20C0171 ) and Korea Centers for Disease Control and Prevention ( 2020-ER6714-00 ), and by a faculty research grant of Yonsei University College of Medicine ( 6-2018-0046 ). Publisher Copyright: © 2021 Japanese Society for Investigative Dermatology

PY - 2021/11

Y1 - 2021/11

N2 - Background: Both human and mouse allergic contact dermatitis (ACD) frequently demonstrates a combined type 1 and type 2 immune response. However, the relative importance of type 2 immunity in this setting has been incompletely understood yet. Objective: To explore an effector function of type 2 immunity in ACD with mixed type 1/type 2 immune response. Methods: Gene expression characteristics of contact hypersensitivity (CHS) model was examined by quantitative polymerase chain reaction. Cytokine profile of T cells was analyzed by flow cytometry. The involvement of type 2 immunity was assessed by antibody-mediated cytokine neutralization and cell depletion. The role of specific subset of cutaneous dendritic cells was evaluated using diphtheria toxin-induced cell-depleting mouse strains. Results: Oxazolone-induced CHS revealed a combination of type 1/type 2 gene expression. The severity of oxazolone-induced CHS was ameliorated by neutralization of IL-4 but not of IFN-γ, indicating that type 2 immunity plays a dominant effector function in this mixed type 1/type 2 model. Mechanistically, type 2 effector immunity was mounted by CD301b+Langeirn− dermal dendritic cells in part through thymic stromal lymphopoietin-interleukin 7 receptor alpha signaling-dependent manner. Conclusion: Our findings suggest the clinical rationale for targeting type 2 immunity as a relevant therapeutic strategy for the mixed immune phenotype of ACD.

AB - Background: Both human and mouse allergic contact dermatitis (ACD) frequently demonstrates a combined type 1 and type 2 immune response. However, the relative importance of type 2 immunity in this setting has been incompletely understood yet. Objective: To explore an effector function of type 2 immunity in ACD with mixed type 1/type 2 immune response. Methods: Gene expression characteristics of contact hypersensitivity (CHS) model was examined by quantitative polymerase chain reaction. Cytokine profile of T cells was analyzed by flow cytometry. The involvement of type 2 immunity was assessed by antibody-mediated cytokine neutralization and cell depletion. The role of specific subset of cutaneous dendritic cells was evaluated using diphtheria toxin-induced cell-depleting mouse strains. Results: Oxazolone-induced CHS revealed a combination of type 1/type 2 gene expression. The severity of oxazolone-induced CHS was ameliorated by neutralization of IL-4 but not of IFN-γ, indicating that type 2 immunity plays a dominant effector function in this mixed type 1/type 2 model. Mechanistically, type 2 effector immunity was mounted by CD301b+Langeirn− dermal dendritic cells in part through thymic stromal lymphopoietin-interleukin 7 receptor alpha signaling-dependent manner. Conclusion: Our findings suggest the clinical rationale for targeting type 2 immunity as a relevant therapeutic strategy for the mixed immune phenotype of ACD.

UR - http://www.scopus.com/inward/record.url?scp=85118839623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85118839623&partnerID=8YFLogxK

U2 - 10.1016/j.jdermsci.2021.10.001

DO - 10.1016/j.jdermsci.2021.10.001

M3 - Article

C2 - 34763990

AN - SCOPUS:85118839623

SN - 0923-1811

VL - 104

SP - 122

EP - 131

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

IS - 2

ER -

Type 2 immunity plays an essential role for murine model of allergic contact dermatitis with mixed type 1/type 2 immune response

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this