Type 2 immunity plays an essential role for murine model of allergic contact dermatitis with mixed type 1/type 2 immune response

Jeyun Park, Jae Won Lee, Sung Hee Kim, Jongwook Oh, Won Seok Roh, Soo Min Kim, Chang Ook Park, Min Geol Lee, Tae Gyun Kim

Research output: Contribution to journalArticlepeer-review


Background: Both human and mouse allergic contact dermatitis (ACD) frequently demonstrates a combined type 1 and type 2 immune response. However, the relative importance of type 2 immunity in this setting has been incompletely understood yet. Objective: To explore an effector function of type 2 immunity in ACD with mixed type 1/type 2 immune response. Methods: Gene expression characteristics of contact hypersensitivity (CHS) model was examined by quantitative polymerase chain reaction. Cytokine profile of T cells was analyzed by flow cytometry. The involvement of type 2 immunity was assessed by antibody-mediated cytokine neutralization and cell depletion. The role of specific subset of cutaneous dendritic cells was evaluated using diphtheria toxin-induced cell-depleting mouse strains. Results: Oxazolone-induced CHS revealed a combination of type 1/type 2 gene expression. The severity of oxazolone-induced CHS was ameliorated by neutralization of IL-4 but not of IFN-γ, indicating that type 2 immunity plays a dominant effector function in this mixed type 1/type 2 model. Mechanistically, type 2 effector immunity was mounted by CD301b+Langeirn− dermal dendritic cells in part through thymic stromal lymphopoietin-interleukin 7 receptor alpha signaling-dependent manner. Conclusion: Our findings suggest the clinical rationale for targeting type 2 immunity as a relevant therapeutic strategy for the mixed immune phenotype of ACD.

Original languageEnglish
Pages (from-to)122-131
Number of pages10
JournalJournal of Dermatological Science
Issue number2
Publication statusPublished - 2021 Nov

Bibliographical note

Funding Information:
This research was supported by a Basic Science Research Program through the National Research Foundation of Republic of Korea funded by the Ministry of Education ( 2019R1A6A1A03032869 ) and Ministry of Science and Information and Communications Technology ( 2019M3A9E8022135, 2020R1C1C1014513 ), by a Korea Health Technology R&D Project through the Korea Health Industry Development Institute funded by the Ministry of Health and Welfare ( HP20C0019, HP20C0171 ) and Korea Centers for Disease Control and Prevention ( 2020-ER6714-00 ), and by a faculty research grant of Yonsei University College of Medicine ( 6-2018-0046 ).

Publisher Copyright:
© 2021 Japanese Society for Investigative Dermatology

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology


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