Type I atelocollagen grafting onto ozone-treated polyurethane films

Cell attachment, proliferation, and collagen synthesis

Jongchul Park, Yu Shik Hwang, Jongeun Lee, Ki Dong Park, Kazuaki Matsumura, Suong Hyu Hyon, Hwal Suh

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

An approach is presented for the graft copolymerization of type I atelocollagen onto the surface of polyurethane (PU) films treated with ozone. Through inducing oxidization to modify PU surface by ozone, peroxide groups are easily generated on the surface. Those peroxides are broken by redox-polymerization, and provide active species which initiate graft polymerization by reacting with amines in the collagen molecules. The ozone oxidation time and voltage could readily control the amount of peroxide production. The surface density of generated peroxides on PU surface was determined by iodide method. The maximum concentration of peroxide was about 10.20 x 10-8mol/cm2 when ozone oxidation was performed at 60 V for 30 min. After the reaction of PU by ozone oxidation, type I atelocollagen was graft-copolymerized onto the PU film. All the physical measurements on the collagen-grafted surface indicated that the PU surface was effectively covered with type I atelocollagen. The interaction of the collagen-grafted PU surface with fibroblasts could be greatly enhanced by the surface graft polymerization with type I atelocollagen. Attachment and proliferation of fibroblasts on the grafted type I atelocollagen were significantly enhanced, and it is assumed that the atelocollagen matrix supported the initial attachment and growth of cells. In the early stage of proliferation, collagen synthesis in fibroblasts was not activated and remained at a relatively low level due to the grafted type I atelocollagen, increasing only with fibroblast differentiation. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish
Pages (from-to)669-677
Number of pages9
JournalJournal of Biomedical Materials Research
Volume52
Issue number4
DOIs
Publication statusPublished - 2000 Dec 1

Fingerprint

Polyurethanes
Ozone
Collagen
Peroxides
Fibroblasts
Grafts
Polymerization
Oxidation
atelocollagen
Iodides
Copolymerization
Amines
Cells
Molecules
Electric potential

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Park, Jongchul ; Hwang, Yu Shik ; Lee, Jongeun ; Park, Ki Dong ; Matsumura, Kazuaki ; Hyon, Suong Hyu ; Suh, Hwal. / Type I atelocollagen grafting onto ozone-treated polyurethane films : Cell attachment, proliferation, and collagen synthesis. In: Journal of Biomedical Materials Research. 2000 ; Vol. 52, No. 4. pp. 669-677.
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Type I atelocollagen grafting onto ozone-treated polyurethane films : Cell attachment, proliferation, and collagen synthesis. / Park, Jongchul; Hwang, Yu Shik; Lee, Jongeun; Park, Ki Dong; Matsumura, Kazuaki; Hyon, Suong Hyu; Suh, Hwal.

In: Journal of Biomedical Materials Research, Vol. 52, No. 4, 01.12.2000, p. 669-677.

Research output: Contribution to journalArticle

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T1 - Type I atelocollagen grafting onto ozone-treated polyurethane films

T2 - Cell attachment, proliferation, and collagen synthesis

AU - Park, Jongchul

AU - Hwang, Yu Shik

AU - Lee, Jongeun

AU - Park, Ki Dong

AU - Matsumura, Kazuaki

AU - Hyon, Suong Hyu

AU - Suh, Hwal

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Y1 - 2000/12/1

N2 - An approach is presented for the graft copolymerization of type I atelocollagen onto the surface of polyurethane (PU) films treated with ozone. Through inducing oxidization to modify PU surface by ozone, peroxide groups are easily generated on the surface. Those peroxides are broken by redox-polymerization, and provide active species which initiate graft polymerization by reacting with amines in the collagen molecules. The ozone oxidation time and voltage could readily control the amount of peroxide production. The surface density of generated peroxides on PU surface was determined by iodide method. The maximum concentration of peroxide was about 10.20 x 10-8mol/cm2 when ozone oxidation was performed at 60 V for 30 min. After the reaction of PU by ozone oxidation, type I atelocollagen was graft-copolymerized onto the PU film. All the physical measurements on the collagen-grafted surface indicated that the PU surface was effectively covered with type I atelocollagen. The interaction of the collagen-grafted PU surface with fibroblasts could be greatly enhanced by the surface graft polymerization with type I atelocollagen. Attachment and proliferation of fibroblasts on the grafted type I atelocollagen were significantly enhanced, and it is assumed that the atelocollagen matrix supported the initial attachment and growth of cells. In the early stage of proliferation, collagen synthesis in fibroblasts was not activated and remained at a relatively low level due to the grafted type I atelocollagen, increasing only with fibroblast differentiation. (C) 2000 John Wiley and Sons, Inc.

AB - An approach is presented for the graft copolymerization of type I atelocollagen onto the surface of polyurethane (PU) films treated with ozone. Through inducing oxidization to modify PU surface by ozone, peroxide groups are easily generated on the surface. Those peroxides are broken by redox-polymerization, and provide active species which initiate graft polymerization by reacting with amines in the collagen molecules. The ozone oxidation time and voltage could readily control the amount of peroxide production. The surface density of generated peroxides on PU surface was determined by iodide method. The maximum concentration of peroxide was about 10.20 x 10-8mol/cm2 when ozone oxidation was performed at 60 V for 30 min. After the reaction of PU by ozone oxidation, type I atelocollagen was graft-copolymerized onto the PU film. All the physical measurements on the collagen-grafted surface indicated that the PU surface was effectively covered with type I atelocollagen. The interaction of the collagen-grafted PU surface with fibroblasts could be greatly enhanced by the surface graft polymerization with type I atelocollagen. Attachment and proliferation of fibroblasts on the grafted type I atelocollagen were significantly enhanced, and it is assumed that the atelocollagen matrix supported the initial attachment and growth of cells. In the early stage of proliferation, collagen synthesis in fibroblasts was not activated and remained at a relatively low level due to the grafted type I atelocollagen, increasing only with fibroblast differentiation. (C) 2000 John Wiley and Sons, Inc.

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