Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation

Daesan Kim; Jaehyuk Lee; Boyoung Kim; Yujin Shin; Jinhong Park; Uijoo Kim; Minbaek Lee; Sang Bum Kim; Sunghoon Kim

doi:10.1039/d2na00227b

Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation

Daesan Kim, Jaehyuk Lee, Boyoung Kim, Yujin Shin, Jinhong Park, Uijoo Kim, Minbaek Lee, Sang Bum Kim, Sunghoon Kim

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

Abstract

Extracellular vesicles (EVs) have emerged as vehicles that mediate diverse cell-cell communication. However, in-depth understanding of these vesicles is hampered by a lack of a reliable isolation method to separate different types of EVs with high levels of integrity and purity. Here, we developed a nanoporous and ultra-thin membrane structure (NUTS) that warrants the size-based isolation of EVs without cake formation, minimizing the sample loss during the filtration process. By utilizing the micro-electro-mechanical systems (MEMS) technique, we could also control the pore size in nanoscale. We validated the performance of this membrane to separate EVs according to their size range.

Original language	English
Pages (from-to)	640-649
Number of pages	10
Journal	Nanoscale Advances
Volume	5
Issue number	3
DOIs	https://doi.org/10.1039/d2na00227b
Publication status	Published - 2022 Nov 22

Bibliographical note

Funding Information:
This research was supported by the Yonsei University Research Fund of 2021-22-0293, 2021-22-0291, and 2021-22-0061; by a grant from the National Research Foundation of Korea (NRF) funded by the Korea Government (MSIT) (No. NRF-2021R1A3B1076605, 2021R1F1A1060166, and 2022R1C1C1013481); and by the Technology Development Program (S2854312) funded by the Ministry of SMEs and Startups (MSS, Korea).

Publisher Copyright:
© 2023 RSC.

All Science Journal Classification (ASJC) codes

Bioengineering
Atomic and Molecular Physics, and Optics
Chemistry(all)
Materials Science(all)
Engineering(all)

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10.1039/d2na00227b

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title = "Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation",

abstract = "Extracellular vesicles (EVs) have emerged as vehicles that mediate diverse cell-cell communication. However, in-depth understanding of these vesicles is hampered by a lack of a reliable isolation method to separate different types of EVs with high levels of integrity and purity. Here, we developed a nanoporous and ultra-thin membrane structure (NUTS) that warrants the size-based isolation of EVs without cake formation, minimizing the sample loss during the filtration process. By utilizing the micro-electro-mechanical systems (MEMS) technique, we could also control the pore size in nanoscale. We validated the performance of this membrane to separate EVs according to their size range.",

author = "Daesan Kim and Jaehyuk Lee and Boyoung Kim and Yujin Shin and Jinhong Park and Uijoo Kim and Minbaek Lee and Kim, {Sang Bum} and Sunghoon Kim",

note = "Funding Information: This research was supported by the Yonsei University Research Fund of 2021-22-0293, 2021-22-0291, and 2021-22-0061; by a grant from the National Research Foundation of Korea (NRF) funded by the Korea Government (MSIT) (No. NRF-2021R1A3B1076605, 2021R1F1A1060166, and 2022R1C1C1013481); and by the Technology Development Program (S2854312) funded by the Ministry of SMEs and Startups (MSS, Korea). Publisher Copyright: {\textcopyright} 2023 RSC.",

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doi = "10.1039/d2na00227b",

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AU - Kim, Daesan

AU - Lee, Jaehyuk

AU - Kim, Boyoung

AU - Shin, Yujin

AU - Park, Jinhong

AU - Kim, Uijoo

AU - Lee, Minbaek

AU - Kim, Sang Bum

AU - Kim, Sunghoon

N1 - Funding Information: This research was supported by the Yonsei University Research Fund of 2021-22-0293, 2021-22-0291, and 2021-22-0061; by a grant from the National Research Foundation of Korea (NRF) funded by the Korea Government (MSIT) (No. NRF-2021R1A3B1076605, 2021R1F1A1060166, and 2022R1C1C1013481); and by the Technology Development Program (S2854312) funded by the Ministry of SMEs and Startups (MSS, Korea). Publisher Copyright: © 2023 RSC.

PY - 2022/11/22

Y1 - 2022/11/22

N2 - Extracellular vesicles (EVs) have emerged as vehicles that mediate diverse cell-cell communication. However, in-depth understanding of these vesicles is hampered by a lack of a reliable isolation method to separate different types of EVs with high levels of integrity and purity. Here, we developed a nanoporous and ultra-thin membrane structure (NUTS) that warrants the size-based isolation of EVs without cake formation, minimizing the sample loss during the filtration process. By utilizing the micro-electro-mechanical systems (MEMS) technique, we could also control the pore size in nanoscale. We validated the performance of this membrane to separate EVs according to their size range.

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Ultra-thin membrane filter with a uniformly arrayed nanopore structure for nanoscale separation of extracellular vesicles without cake formation

Abstract

Bibliographical note

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