Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system

Sung Ho Lee; Bumjoon Jang; Dong Hee Kim; Chang Hyun Park; Gyuri Bae; Seung Woo Park; Seung Han Park

doi:10.1117/12.2270692

Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system

Sung Ho Lee, Bumjoon Jang, Dong Hee Kim, Chang Hyun Park, Gyuri Bae, Seung Woo Park, Seung Han Park

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Unlike those of other ordinary laser scanning microscopies in the past, nonlinear optical laser scanning microscopy (SHG, THG microscopy) applied ultrafast laser technology which has high peak powers with relatively inexpensive, low-average-power. It short pulse nature reduces the ionization damage in organic molecules. And it enables us to take bright label-free images. In this study, we measured cell division of zebrafish egg with ultrafast video images using multimodal nonlinear optical microscope. The result shows in-vivo cell division label-free imaging with sub-cellular resolution.

Original language	English
Title of host publication	International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
Editors	Jaebum Choo, Seung-Han Park
Publisher	SPIE
ISBN (Electronic)	9781510610934
DOIs	https://doi.org/10.1117/12.2270692
Publication status	Published - 2017
Event	International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017 - Jeju, Korea, Republic of Duration: 2017 Feb 22 → 2017 Feb 24

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10324
ISSN (Print)	1605-7422

Other

Other	International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
Country/Territory	Korea, Republic of
City	Jeju
Period	17/2/22 → 17/2/24

Bibliographical note

Publisher Copyright:
© 2017 SPIE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2270692

Cite this

Lee, S. H., Jang, B., Kim, D. H., Park, C. H., Bae, G., Park, S. W., & Park, S. H. (2017). Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system. In J. Choo, & S-H. Park (Eds.), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017 [103240B] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10324). SPIE. https://doi.org/10.1117/12.2270692

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abstract = "Unlike those of other ordinary laser scanning microscopies in the past, nonlinear optical laser scanning microscopy (SHG, THG microscopy) applied ultrafast laser technology which has high peak powers with relatively inexpensive, low-average-power. It short pulse nature reduces the ionization damage in organic molecules. And it enables us to take bright label-free images. In this study, we measured cell division of zebrafish egg with ultrafast video images using multimodal nonlinear optical microscope. The result shows in-vivo cell division label-free imaging with sub-cellular resolution.",

author = "Lee, {Sung Ho} and Bumjoon Jang and Kim, {Dong Hee} and Park, {Chang Hyun} and Gyuri Bae and Park, {Seung Woo} and Park, {Seung Han}",

note = "Publisher Copyright: {\textcopyright} 2017 SPIE.; International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017 ; Conference date: 22-02-2017 Through 24-02-2017",

year = "2017",

doi = "10.1117/12.2270692",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Lee, SH, Jang, B, Kim, DH, Park, CH, Bae, G, Park, SW & Park, SH 2017, Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system. in J Choo & S-H Park (eds), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017., 103240B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10324, SPIE, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, Jeju, Korea, Republic of, 17/2/22. https://doi.org/10.1117/12.2270692

Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system. / Lee, Sung Ho; Jang, Bumjoon; Kim, Dong Hee et al.

International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017. ed. / Jaebum Choo; Seung-Han Park. SPIE, 2017. 103240B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10324).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Lee, Sung Ho

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AU - Park, Seung Woo

AU - Park, Seung Han

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N2 - Unlike those of other ordinary laser scanning microscopies in the past, nonlinear optical laser scanning microscopy (SHG, THG microscopy) applied ultrafast laser technology which has high peak powers with relatively inexpensive, low-average-power. It short pulse nature reduces the ionization damage in organic molecules. And it enables us to take bright label-free images. In this study, we measured cell division of zebrafish egg with ultrafast video images using multimodal nonlinear optical microscope. The result shows in-vivo cell division label-free imaging with sub-cellular resolution.

AB - Unlike those of other ordinary laser scanning microscopies in the past, nonlinear optical laser scanning microscopy (SHG, THG microscopy) applied ultrafast laser technology which has high peak powers with relatively inexpensive, low-average-power. It short pulse nature reduces the ionization damage in organic molecules. And it enables us to take bright label-free images. In this study, we measured cell division of zebrafish egg with ultrafast video images using multimodal nonlinear optical microscope. The result shows in-vivo cell division label-free imaging with sub-cellular resolution.

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M3 - Conference contribution

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017

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Lee SH, Jang B, Kim DH, Park CH, Bae G, Park SW et al. Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system. In Choo J, Park S-H, editors, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017. SPIE. 2017. 103240B. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2270692

Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system

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