Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system

Sung Ho Lee, Bumjoon Jang, Dong Hee Kim, Chang Hyun Park, Gyuri Bae, Seung Woo Park, Seung Han Park

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Unlike those of other ordinary laser scanning microscopies in the past, nonlinear optical laser scanning microscopy (SHG, THG microscopy) applied ultrafast laser technology which has high peak powers with relatively inexpensive, low-average-power. It short pulse nature reduces the ionization damage in organic molecules. And it enables us to take bright label-free images. In this study, we measured cell division of zebrafish egg with ultrafast video images using multimodal nonlinear optical microscope. The result shows in-vivo cell division label-free imaging with sub-cellular resolution.

Original languageEnglish
Title of host publicationInternational Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
EditorsJaebum Choo, Seung-Han Park
PublisherSPIE
Volume10324
ISBN (Electronic)9781510610934
DOIs
Publication statusPublished - 2017 Jan 1
EventInternational Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017 - Jeju, Korea, Republic of
Duration: 2017 Feb 222017 Feb 24

Other

OtherInternational Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017
CountryKorea, Republic of
CityJeju
Period17/2/2217/2/24

Fingerprint

cell division
eggs
Zebrafish
Confocal Microscopy
Cell Division
Ovum
Microscopic examination
Cells
microscopy
Imaging techniques
Labels
lasers
Scanning
Microscopy
Ultrafast lasers
Lasers
scanning
Technology
optical microscopes
Ionization

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Lee, S. H., Jang, B., Kim, D. H., Park, C. H., Bae, G., Park, S. W., & Park, S. H. (2017). Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system. In J. Choo, & S-H. Park (Eds.), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017 (Vol. 10324). [103240B] SPIE. https://doi.org/10.1117/12.2270692
Lee, Sung Ho ; Jang, Bumjoon ; Kim, Dong Hee ; Park, Chang Hyun ; Bae, Gyuri ; Park, Seung Woo ; Park, Seung Han. / Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system. International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017. editor / Jaebum Choo ; Seung-Han Park. Vol. 10324 SPIE, 2017.
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Lee, SH, Jang, B, Kim, DH, Park, CH, Bae, G, Park, SW & Park, SH 2017, Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system. in J Choo & S-H Park (eds), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017. vol. 10324, 103240B, SPIE, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, Jeju, Korea, Republic of, 17/2/22. https://doi.org/10.1117/12.2270692

Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system. / Lee, Sung Ho; Jang, Bumjoon; Kim, Dong Hee; Park, Chang Hyun; Bae, Gyuri; Park, Seung Woo; Park, Seung Han.

International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017. ed. / Jaebum Choo; Seung-Han Park. Vol. 10324 SPIE, 2017. 103240B.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - Unlike those of other ordinary laser scanning microscopies in the past, nonlinear optical laser scanning microscopy (SHG, THG microscopy) applied ultrafast laser technology which has high peak powers with relatively inexpensive, low-average-power. It short pulse nature reduces the ionization damage in organic molecules. And it enables us to take bright label-free images. In this study, we measured cell division of zebrafish egg with ultrafast video images using multimodal nonlinear optical microscope. The result shows in-vivo cell division label-free imaging with sub-cellular resolution.

AB - Unlike those of other ordinary laser scanning microscopies in the past, nonlinear optical laser scanning microscopy (SHG, THG microscopy) applied ultrafast laser technology which has high peak powers with relatively inexpensive, low-average-power. It short pulse nature reduces the ionization damage in organic molecules. And it enables us to take bright label-free images. In this study, we measured cell division of zebrafish egg with ultrafast video images using multimodal nonlinear optical microscope. The result shows in-vivo cell division label-free imaging with sub-cellular resolution.

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Lee SH, Jang B, Kim DH, Park CH, Bae G, Park SW et al. Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system. In Choo J, Park S-H, editors, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017. Vol. 10324. SPIE. 2017. 103240B https://doi.org/10.1117/12.2270692