The detection sensitivity of surface plasmon resonance (SPR) biosensors has been improved by employing colocalization of spatial distribution of electromagnetic near-fields and detection molecules. We have used plasmon nanolithography to achieve light-matter colocalization on triangular nanoaperture arrays and optimized array configurations to improve colocalization efficiency. Streptavidin-biotin interactions were measured to validate the concept. It was confirmed that colocalized distributions of target binding and localized near-fields produced larger optical detection sensitivity. The colocalized detection was also shown to come with wider dynamic range than noncolocalized detection. The effective limit-of-detection of colocalized measurements was on the order of 30 pM. The colocalized detection sensitivity was estimated to be below 1 fg/mm2 in a 100-nm deep evanescent area, an enhancement by more than three orders of magnitude over conventional SPR sensor.
|Title of host publication||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV|
|Editors||Dan V. Nicolau, Alexander N. Cartwright, Dror Fixler|
|Publication status||Published - 2018|
|Event||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV 2018 - San Francisco, United States|
Duration: 2018 Jan 30 → 2018 Jan 31
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Conference||Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV 2018|
|Period||18/1/30 → 18/1/31|
Bibliographical notePublisher Copyright:
© 2018 SPIE.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging