Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields

Kiheung Kim, Wonju Lee, Kyungwha Chung, Hongki Lee, Taehwang Son, Youngjin Oh, Yun Feng Xiao, Dong Ha Kim, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The detection sensitivity of surface plasmon resonance (SPR) biosensors has been improved by employing colocalization of spatial distribution of electromagnetic near-fields and detection molecules. We have used plasmon nanolithography to achieve light-matter colocalization on triangular nanoaperture arrays and optimized array configurations to improve colocalization efficiency. Streptavidin-biotin interactions were measured to validate the concept. It was confirmed that colocalized distributions of target binding and localized near-fields produced larger optical detection sensitivity. The colocalized detection was also shown to come with wider dynamic range than noncolocalized detection. The effective limit-of-detection of colocalized measurements was on the order of 30 pM. The colocalized detection sensitivity was estimated to be below 1 fg/mm2 in a 100-nm deep evanescent area, an enhancement by more than three orders of magnitude over conventional SPR sensor.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV
EditorsDan V. Nicolau, Alexander N. Cartwright, Dror Fixler
PublisherSPIE
ISBN (Electronic)9781510614970
DOIs
Publication statusPublished - 2018 Jan 1
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV 2018 - San Francisco, United States
Duration: 2018 Jan 302018 Jan 31

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10506
ISSN (Print)1605-7422

Conference

ConferenceNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV 2018
CountryUnited States
CitySan Francisco
Period18/1/3018/1/31

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Kim, K., Lee, W., Chung, K., Lee, H., Son, T., Oh, Y., Xiao, Y. F., Kim, D. H., & Kim, D. (2018). Ultrasensitive colocalization detection based on plasmonic nanolithography with molecular-overlapped optical near-fields. In D. V. Nicolau, A. N. Cartwright, & D. Fixler (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV [1050610] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10506). SPIE. https://doi.org/10.1117/12.2291449