Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba histolytica.

Seobo Sim, Kyeong Ah Kim, Taisoon Yong, Soon Jung Park, Kyung il Im, Myeong Heon Shin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Neutrophils are important effector cells against protozoan extracellular parasite Entamoeba histolytica, which causes amoebic colitis and liver abscess in human beings. Apoptotic cell death of neutrophils is an important event in the resolution of inflammation and parasite's survival in vivo. This study was undertaken to investigate the ultrastructural aspects of apoptotic cells during neutrophil death triggered by Entamoeba histolytica. Isolated human neutrophils from the peripheral blood were incubated with or without live trophozoites of E. histolytica and examined by transmission electron microscopy (TEM). Neutrophils incubated with E. histolytica were observed to show apoptotic characteristics, such as compaction of the nuclear chromatin and swelling of the nuclear envelop. In contrast, neutrophils incubated in the absence of the amoeba had many protrusions of irregular cell surfaces and heterogenous nuclear chromatin. Therefore, it is suggested that Entamoeba-induced neutrophil apoptosis contribute to prevent unwanted tissue inflammation and damage in the amoeba-invaded lesions in vivo.

Original languageEnglish
Pages (from-to)205-208
Number of pages4
JournalThe Korean journal of parasitology
Volume42
Issue number4
DOIs
Publication statusPublished - 2004 Jan 1

Fingerprint

Entamoeba histolytica
Neutrophils
Cell Death
Observation
Amoeba
Chromatin
Parasites
Cell Surface Extensions
Entamoeba
Amoebic Dysentery
Amoebic Liver Abscess
Inflammation
Trophozoites
Transmission Electron Microscopy
Apoptosis

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Sim, Seobo ; Kim, Kyeong Ah ; Yong, Taisoon ; Park, Soon Jung ; Im, Kyung il ; Shin, Myeong Heon. / Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba histolytica. In: The Korean journal of parasitology. 2004 ; Vol. 42, No. 4. pp. 205-208.
@article{a8eea18d75ff4b158e8d6720f7b6977e,
title = "Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba histolytica.",
abstract = "Neutrophils are important effector cells against protozoan extracellular parasite Entamoeba histolytica, which causes amoebic colitis and liver abscess in human beings. Apoptotic cell death of neutrophils is an important event in the resolution of inflammation and parasite's survival in vivo. This study was undertaken to investigate the ultrastructural aspects of apoptotic cells during neutrophil death triggered by Entamoeba histolytica. Isolated human neutrophils from the peripheral blood were incubated with or without live trophozoites of E. histolytica and examined by transmission electron microscopy (TEM). Neutrophils incubated with E. histolytica were observed to show apoptotic characteristics, such as compaction of the nuclear chromatin and swelling of the nuclear envelop. In contrast, neutrophils incubated in the absence of the amoeba had many protrusions of irregular cell surfaces and heterogenous nuclear chromatin. Therefore, it is suggested that Entamoeba-induced neutrophil apoptosis contribute to prevent unwanted tissue inflammation and damage in the amoeba-invaded lesions in vivo.",
author = "Seobo Sim and Kim, {Kyeong Ah} and Taisoon Yong and Park, {Soon Jung} and Im, {Kyung il} and Shin, {Myeong Heon}",
year = "2004",
month = "1",
day = "1",
doi = "10.3347/kjp.2004.42.4.205",
language = "English",
volume = "42",
pages = "205--208",
journal = "Korean Journal of Parasitology",
issn = "0023-4001",
publisher = "Korean Journal of Parasitology",
number = "4",

}

Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba histolytica. / Sim, Seobo; Kim, Kyeong Ah; Yong, Taisoon; Park, Soon Jung; Im, Kyung il; Shin, Myeong Heon.

In: The Korean journal of parasitology, Vol. 42, No. 4, 01.01.2004, p. 205-208.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ultrastructural observation of human neutrophils during apoptotic cell death triggered by Entamoeba histolytica.

AU - Sim, Seobo

AU - Kim, Kyeong Ah

AU - Yong, Taisoon

AU - Park, Soon Jung

AU - Im, Kyung il

AU - Shin, Myeong Heon

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Neutrophils are important effector cells against protozoan extracellular parasite Entamoeba histolytica, which causes amoebic colitis and liver abscess in human beings. Apoptotic cell death of neutrophils is an important event in the resolution of inflammation and parasite's survival in vivo. This study was undertaken to investigate the ultrastructural aspects of apoptotic cells during neutrophil death triggered by Entamoeba histolytica. Isolated human neutrophils from the peripheral blood were incubated with or without live trophozoites of E. histolytica and examined by transmission electron microscopy (TEM). Neutrophils incubated with E. histolytica were observed to show apoptotic characteristics, such as compaction of the nuclear chromatin and swelling of the nuclear envelop. In contrast, neutrophils incubated in the absence of the amoeba had many protrusions of irregular cell surfaces and heterogenous nuclear chromatin. Therefore, it is suggested that Entamoeba-induced neutrophil apoptosis contribute to prevent unwanted tissue inflammation and damage in the amoeba-invaded lesions in vivo.

AB - Neutrophils are important effector cells against protozoan extracellular parasite Entamoeba histolytica, which causes amoebic colitis and liver abscess in human beings. Apoptotic cell death of neutrophils is an important event in the resolution of inflammation and parasite's survival in vivo. This study was undertaken to investigate the ultrastructural aspects of apoptotic cells during neutrophil death triggered by Entamoeba histolytica. Isolated human neutrophils from the peripheral blood were incubated with or without live trophozoites of E. histolytica and examined by transmission electron microscopy (TEM). Neutrophils incubated with E. histolytica were observed to show apoptotic characteristics, such as compaction of the nuclear chromatin and swelling of the nuclear envelop. In contrast, neutrophils incubated in the absence of the amoeba had many protrusions of irregular cell surfaces and heterogenous nuclear chromatin. Therefore, it is suggested that Entamoeba-induced neutrophil apoptosis contribute to prevent unwanted tissue inflammation and damage in the amoeba-invaded lesions in vivo.

UR - http://www.scopus.com/inward/record.url?scp=16544393116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16544393116&partnerID=8YFLogxK

U2 - 10.3347/kjp.2004.42.4.205

DO - 10.3347/kjp.2004.42.4.205

M3 - Article

VL - 42

SP - 205

EP - 208

JO - Korean Journal of Parasitology

JF - Korean Journal of Parasitology

SN - 0023-4001

IS - 4

ER -