Ultraviolet photolithographic development of polyphosphazene hydrogel microstructures for potential use in microarray biosensors

Harry R. Allcock, Mwita V.B. Phelps, Eric W. Barrett, Michael V. Pishko, Won Gun Koh

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Polyphosphazenes that bear both methoxyethoxyethoxy and cinnamyl side groups were synthesized and evaluated for use as hydrogels incorporated into micrometer-scale biosensor arrays. Polymers with the general formula [NPR xR′ y] n where R = OCH 2CH 2OCH 2CH 2OCH 3 and R′ = OCH=CHCH 2Ph (x = y = 1; x = 2, y = 0) were synthesized. The polymers were cross-linked to form hydrogels by exposure to ultraviolet radiation (λ = 320-480 nm) in the presence of a photoinitiator. Hydrogel microstructures in the size range 50-500 μm were fabricated using standard photolithographic techniques. The resolution and dimensions of these microstructures were examined by optical microscopy, scanning electron microscopy, and profilometry. The resultant three-dimensional hydrogel microstructures were used to encapsulate enzymes for biosensor applications. The enzymatic activity of encapsulated horseradish peroxidase (HRP) was examined as a model system. The HRP catalyzed reaction between H 2O 2 and Amplex Red to produce a fluorescent product, resorufin, was confirmed by fluorescence microscopy.

Original languageEnglish
Pages (from-to)609-613
Number of pages5
JournalChemistry of Materials
Volume18
Issue number3
DOIs
Publication statusPublished - 2006 Feb 7

Fingerprint

Hydrogel
Microarrays
Biosensors
Hydrogels
Horseradish Peroxidase
Microstructure
Polymers
Profilometry
Fluorescence microscopy
Ultraviolet radiation
Optical microscopy
Scanning electron microscopy
Enzymes
poly(phosphazene)

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

Cite this

Allcock, Harry R. ; Phelps, Mwita V.B. ; Barrett, Eric W. ; Pishko, Michael V. ; Koh, Won Gun. / Ultraviolet photolithographic development of polyphosphazene hydrogel microstructures for potential use in microarray biosensors. In: Chemistry of Materials. 2006 ; Vol. 18, No. 3. pp. 609-613.
@article{13ddbf3f434943b5bc2ed3f79f86701c,
title = "Ultraviolet photolithographic development of polyphosphazene hydrogel microstructures for potential use in microarray biosensors",
abstract = "Polyphosphazenes that bear both methoxyethoxyethoxy and cinnamyl side groups were synthesized and evaluated for use as hydrogels incorporated into micrometer-scale biosensor arrays. Polymers with the general formula [NPR xR′ y] n where R = OCH 2CH 2OCH 2CH 2OCH 3 and R′ = OCH=CHCH 2Ph (x = y = 1; x = 2, y = 0) were synthesized. The polymers were cross-linked to form hydrogels by exposure to ultraviolet radiation (λ = 320-480 nm) in the presence of a photoinitiator. Hydrogel microstructures in the size range 50-500 μm were fabricated using standard photolithographic techniques. The resolution and dimensions of these microstructures were examined by optical microscopy, scanning electron microscopy, and profilometry. The resultant three-dimensional hydrogel microstructures were used to encapsulate enzymes for biosensor applications. The enzymatic activity of encapsulated horseradish peroxidase (HRP) was examined as a model system. The HRP catalyzed reaction between H 2O 2 and Amplex Red to produce a fluorescent product, resorufin, was confirmed by fluorescence microscopy.",
author = "Allcock, {Harry R.} and Phelps, {Mwita V.B.} and Barrett, {Eric W.} and Pishko, {Michael V.} and Koh, {Won Gun}",
year = "2006",
month = "2",
day = "7",
doi = "10.1021/cm050316b",
language = "English",
volume = "18",
pages = "609--613",
journal = "Chemistry of Materials",
issn = "0897-4756",
publisher = "American Chemical Society",
number = "3",

}

Ultraviolet photolithographic development of polyphosphazene hydrogel microstructures for potential use in microarray biosensors. / Allcock, Harry R.; Phelps, Mwita V.B.; Barrett, Eric W.; Pishko, Michael V.; Koh, Won Gun.

In: Chemistry of Materials, Vol. 18, No. 3, 07.02.2006, p. 609-613.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ultraviolet photolithographic development of polyphosphazene hydrogel microstructures for potential use in microarray biosensors

AU - Allcock, Harry R.

AU - Phelps, Mwita V.B.

AU - Barrett, Eric W.

AU - Pishko, Michael V.

AU - Koh, Won Gun

PY - 2006/2/7

Y1 - 2006/2/7

N2 - Polyphosphazenes that bear both methoxyethoxyethoxy and cinnamyl side groups were synthesized and evaluated for use as hydrogels incorporated into micrometer-scale biosensor arrays. Polymers with the general formula [NPR xR′ y] n where R = OCH 2CH 2OCH 2CH 2OCH 3 and R′ = OCH=CHCH 2Ph (x = y = 1; x = 2, y = 0) were synthesized. The polymers were cross-linked to form hydrogels by exposure to ultraviolet radiation (λ = 320-480 nm) in the presence of a photoinitiator. Hydrogel microstructures in the size range 50-500 μm were fabricated using standard photolithographic techniques. The resolution and dimensions of these microstructures were examined by optical microscopy, scanning electron microscopy, and profilometry. The resultant three-dimensional hydrogel microstructures were used to encapsulate enzymes for biosensor applications. The enzymatic activity of encapsulated horseradish peroxidase (HRP) was examined as a model system. The HRP catalyzed reaction between H 2O 2 and Amplex Red to produce a fluorescent product, resorufin, was confirmed by fluorescence microscopy.

AB - Polyphosphazenes that bear both methoxyethoxyethoxy and cinnamyl side groups were synthesized and evaluated for use as hydrogels incorporated into micrometer-scale biosensor arrays. Polymers with the general formula [NPR xR′ y] n where R = OCH 2CH 2OCH 2CH 2OCH 3 and R′ = OCH=CHCH 2Ph (x = y = 1; x = 2, y = 0) were synthesized. The polymers were cross-linked to form hydrogels by exposure to ultraviolet radiation (λ = 320-480 nm) in the presence of a photoinitiator. Hydrogel microstructures in the size range 50-500 μm were fabricated using standard photolithographic techniques. The resolution and dimensions of these microstructures were examined by optical microscopy, scanning electron microscopy, and profilometry. The resultant three-dimensional hydrogel microstructures were used to encapsulate enzymes for biosensor applications. The enzymatic activity of encapsulated horseradish peroxidase (HRP) was examined as a model system. The HRP catalyzed reaction between H 2O 2 and Amplex Red to produce a fluorescent product, resorufin, was confirmed by fluorescence microscopy.

UR - http://www.scopus.com/inward/record.url?scp=32944456140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32944456140&partnerID=8YFLogxK

U2 - 10.1021/cm050316b

DO - 10.1021/cm050316b

M3 - Article

AN - SCOPUS:32944456140

VL - 18

SP - 609

EP - 613

JO - Chemistry of Materials

JF - Chemistry of Materials

SN - 0897-4756

IS - 3

ER -