Ultraviolet photolithographic development of polyphosphazene hydrogel microstructures for potential use in microarray biosensors

Harry R. Allcock, Mwita V.B. Phelps, Eric W. Barrett, Michael V. Pishko, Won Gun Koh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Polyphosphazenes that bear both methoxyethoxyethoxy and cinnamyl side groups were synthesized and evaluated for use as hydrogels incorporated into micrometer-scale biosensor arrays. Polymers with the general formula [NPR xR′ y] n where R = OCH 2CH 2OCH 2CH 2OCH 3 and R′ = OCH=CHCH 2Ph (x = y = 1; x = 2, y = 0) were synthesized. The polymers were cross-linked to form hydrogels by exposure to ultraviolet radiation (λ = 320-480 nm) in the presence of a photoinitiator. Hydrogel microstructures in the size range 50-500 μm were fabricated using standard photolithographic techniques. The resolution and dimensions of these microstructures were examined by optical microscopy, scanning electron microscopy, and profilometry. The resultant three-dimensional hydrogel microstructures were used to encapsulate enzymes for biosensor applications. The enzymatic activity of encapsulated horseradish peroxidase (HRP) was examined as a model system. The HRP catalyzed reaction between H 2O 2 and Amplex Red to produce a fluorescent product, resorufin, was confirmed by fluorescence microscopy.

Original languageEnglish
Pages (from-to)609-613
Number of pages5
JournalChemistry of Materials
Volume18
Issue number3
DOIs
Publication statusPublished - 2006 Feb 7

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

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